玉米赤霉烯酮间接竞争ELISA方法的建立

Establishment of an Indirect Competitive ELISA for Zearalenone

摘要: 本研究以玉米面为例，建立了玉米赤霉烯酮间接竞争ELISA（ic-ELISA）方法。采用棋盘滴定法确定抗原和单抗的最佳工作浓度，并确定了抗原37 ℃包被1 h、不封闭、酶催化底物作用时间15 min的反应条件进行检测。该方法的检测范围（IC₂₀~IC₈₀）为11~292 pg/mL，检测限（IC₁₀）为6 pg/mL。玉米赤霉烯酮在玉米面中的加标回收率为81.29%~105.80%。本研究建立的ic-ELISA方法与赭曲霉素A、黄曲霉素B₁、呕吐毒素、伏马毒素B₁、T-2毒素无交叉反应，可用于玉米面中玉米赤霉烯酮的初筛检测。

Abstract: In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action time of 15 min were determined the optimal conditions. The results showed that the detection range (IC₂₀~IC₈₀) was 11~292 pg/mL, and the minimum detection limit (IC₁₀) was 6 pg/mL. The recoveries of the zearalenone from corn flour were 81.29%~105.80%. There was no cross reaction with ochratoxin A, aflatoxin B₁, deoxynivalenol, fumonisin B₁, T-2 toxin. The ic-ELISA could be used for detection of zearalenon in corn flour.