有氧运动联合补充D-核糖对小鼠抗疲劳和抗氧化的作用

张振刚; 王峰

doi:10.13386/j.issn1002-0306.2021050117

有氧运动联合补充D-核糖对小鼠抗疲劳和抗氧化的作用

张振刚,
王峰

Effects of Aerobic Exercise Combined with D-Ribose Supplementation on Anti-fatigue and Anti-oxidation in Mice

摘要

摘要: 研究有氧运动联合补充D-核糖对小鼠抗疲劳和抗氧化酶活性的影响，将小鼠分为安静对照组、有氧运动对照组、D-核糖对照组、D-核糖+有氧运动组。有氧运动组进行每周进行6 d的有氧跑台训练，D-核糖组每日每只灌胃2 mL核糖（300 mg/100 g），通过12周的规律性有氧跑台训练后，建立小鼠负重游泳抗疲劳动物模型，分别记录小鼠负重力竭游泳时间，并测定小鼠体内肝糖原（hepaticr glycogen，HG）、肌糖原（muscle glycogen，MG）、血糖（blood glucose）、血尿素氮（blood urea nitrogen，BUN）、血乳酸( bloodlactic acid，BLA）、丙二醛（malondialdehyde，MDA）含量和总抗氧化能力（total antioxidant capacity，T-AOC）、谷胱甘肽过氧化物歧化酶（glutathione peroxide dismutase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）等活力。结果表明，与安静对照组相比，有氧运动对照组、D-核糖对照组、D-核糖+有氧运动组小鼠负重游泳时间显著增加（P<0.05），同时显著提高了小鼠体内肝糖原、肌糖原和血糖含量（P<0.05），并降低了体内血尿素氮和血乳酸含量（P<0.05）。另外，其抗氧化活性结果表明，与安静对照组相比，有氧运动对照组、D-核糖对照组、D-核糖+有氧运动组的T-AOC均增强，SOD、CAT、GSH-Px活性也都显著升高（P<0.05），MDA则显著降低（P<0.05）。说明长期规律的有氧运动联合补充D-核糖能显著提高机体内肝糖原、肌糖原、血糖含量和氧化应激能力，提高机体抗疲劳作用。

Abstract: Study the effects of aerobic exercise combined with D-ribose supplementation on the anti-fatigue and antioxidant enzyme activities of mice, the mice were divided into quiet control group, aerobic exercise control group, D-ribose control group, D-ribose+aerobic exercise group. The aerobic exercise group received aerobic treadmill training for 6 days a week, and the D-ribose group was given 2 mL of ribose (300 mg/100 g) per rat per day. After 12 weeks of regular aerobic treadmill training, an anti-fatigue animal model was established for mice swimming with weight, the time of exhausted swimming was recorded in mice, and hepaticr glycogen (HG), muscle glycogen (MG), and blood glucose in mice, blood urea nitrogen (BUN), blood lactic acid (BLA), malondialdehyde (MDA) content and total antioxidant capacity (T-AOC), glutathione Oxide dismutase (glutathione peroxide dismutase, GSH-Px), superoxide dismutase (SOD), catalase (catalase, CAT) and other activities were measured. The results showed that compared with the quiet control group, the weight-bearing swimming time of mice in the aerobic exercise control group, D-ribose control group, and D-ribose+aerobic exercise group significantly increased (P<0.05), and at the same time the mice's body hepatic glycogen, muscle glycogen and blood sugar content were significantly improved(P<0.05), and the blood urea nitrogen and blood lactic acid content in the body were significantly reduced(P<0.05). In addition, the results of its antioxidant activity showed that compared with the quiet control group, the T-AOC of the aerobic exercise control group, D-ribose control group, D-ribose+aerobic exercise group were all enhanced. The activity of SOD, CAT, GSH-Px also significantly increased (P<0.05), and MDA was significantly reduced (P<0.05). It showed that long-term regular aerobic exercise combined with D-ribose supplementation could significantly increase the liver glycogen, muscle glycogen, blood sugar content and oxidative stress ability in the body, and improve the body's anti-fatigue effect.