Abstract: A method was established for the detection of nonylphenol migration in instant noodle barrel by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). Using this method, we conduct risk analysis and assessment to marketed instant noodle barrels. The chromatographic separation and solid phase extraction conditions were optimized. The calibration curves of the nonylphenol showed good linear relationship in the range of 1~500 μg/L. The limits of detection ranged from 0.002~0.004 μg/L. The recoveries of real samples migration fluid at different spiked levels were in the range of 79.8%~105.2%, and the relative standard deviations (RSDs) were 1.74%~9.88%. 54 batches commercial instant noodle barrel were determined with this method, 45 samples detected nonylphenol, and nonylphenol content was 0.006~0.079 µg/L. According to the test results, the safety of nonyphenol in the instant noodle barrel was analyzed. The maximum daily intake of nonyphenol was 0.0237 µg/d, which was far lower than the health guidance value of nonyphenol (3 µg/d), indicating that the instant noodle barrel in the market is relatively safe and has a low risk to human health.