L-茶氨酸与表没食子儿茶素没食子酸酯预防肥胖及高胆固醇血症的协同调节作用

刘宝贵; 陈致印; 张杨玲; 刘恩硕; 韩淑敏; 肖文军

doi:10.13386/j.issn1002-0306.2021050056

L-茶氨酸与表没食子儿茶素没食子酸酯预防肥胖及高胆固醇血症的协同调节作用

Synergistic Moderating Effects of L-Theanine and EGCG for the Prevention of Obesity and Hypercholesterolemia

摘要

摘要: 以成功造模肥胖及高胆固醇血症大鼠的高脂饲料为基础，以辛伐他汀为阳性对照，比较研究了表没食子儿茶素没食子酸酯（EGCG，83.3 mg·kg⁻¹·d⁻¹）+L-茶氨酸（16.7 mg·kg⁻¹·d⁻¹）、EGCG（100 mg·kg⁻¹·d⁻¹）和L-茶氨酸（100 mg·kg⁻¹·d⁻¹）连续灌胃高脂饮食SPF级SD大鼠56 d，以探究L-茶氨酸及EGCG预防肥胖及高胆固醇血症的协同调节作用。结果表明，与正常组相比，高脂饮食组大鼠体重和血脂水平均有显著差异（P<0.05），且体重已超过正常组体重的21.2%，血清总胆固醇超过正常组的79.9%，说明肥胖及高胆固醇血症模型大鼠造模成功；与模型组相比，所有干预组均能有效抑制长期高脂饮食大鼠体重的增长及血脂水平的升高；与L-茶氨酸组相比，EGCG+L-茶氨酸联合干预组体重、血脂、肝脂以及肝脏氧化还原状态均显著改善（P<0.05）；与EGCG组相比，EGCG+L-茶氨酸联合干预组在体重、腹腔脂肪质量、肝脏系数、器官指数、肝脏丙二醛含量、肝脏过氧化氢酶活性、血清载脂蛋白B100含量、载脂蛋白B100/A1比值以及肝脏脂质积累方面表现出更好的作用效果；与辛伐他汀组相比，只有EGCG+L-茶氨酸联合干预组的体重、腹腔脂肪质量以及肝脏TG含量显著降低（P<0.05）；同时病理切片显示，长期高脂饮食所致肥胖及高胆固醇血症大鼠的组织和器官脂质大量积聚，而EGCG+L-茶氨酸联合干预组则明显改善。由此说明，L-茶氨酸及EGCG在预防肥胖及高胆固醇血症方面具有协同调节作用。

Abstract: Based on the high-fat diet of successfully modeled obese and hypercholesterolemic rats, using simvastatin as a positive control, the results of epigallocatechin gallate (EGCG, 83.3 mg·kg⁻¹·d⁻¹) + L-Theanine (16.7 mg·kg⁻¹·d⁻¹), EGCG (100 mg·kg⁻¹·d⁻¹) and L-theanine (100 mg·kg⁻¹·d⁻¹) continuously fed by gavage to high-fat diet SPF-grade SD rats for 56 d were compared to investigate the synergistic moderating effects of L-theanine and EGCG for the prevention of obesity and hypercholesterolemia. The results showed that compared with the normal group, there were significant differences (P<0.05) in body weight and blood lipid level in the high-fat diet group, and the body weight had exceeded 21.2% of the weight of the normal group, and the total serum cholesterol exceeded 79.9% of the normal group, indicating that the obesity and hypercholesterolemia model rats were successfully modeled. Compared with the model group, all intervention groups could effectively inhibit the increase of body weight and blood lipid level in rats on long-term high-fat diet; compared with the L-theanine group, the combined EGCG+L-theanine intervention group significantly improved body weight, blood lipid, liver lipid and liver redox status (P<0.05). The combined EGCG+L-theanine intervention group showed better effects on body weight, abdominal fat mass, liver coefficient, organ index, hepatic malondialdehyde content, hepatic peroxidase activity, serum Apo B100 content, Apo B100/A1 ratio, and hepatic lipid accumulation compared to the EGCG group. Compared with the simvastatin group, only the combined EGCG+L-theanine intervention group showed a significant reduction in body weight, abdominal fat mass, and liver TG content (P<0.05); meanwhile, the pathological sections showed a large accumulation of lipids in tissues and organs of rats with long-term high-fat diet-induced obesity and hypercholesterolemia, which was significantly improved in the combined EGCG+L-theanine intervention group. This suggests that L-theanine and EGCG have synergistic regulatory effects in the prevention of obesity and hypercholesterolemia.