葡萄籽油对急性肝损伤的保护作用

董颖; 陈鑫沛; 杨浩铎; 王莹莹; 王东营; 汪学德

doi:10.13386/j.issn1002-0306.2021040247

葡萄籽油对急性肝损伤的保护作用

Hepatoprotective Effects of Grape Seed Oil on Acute Liver Injury

摘要

摘要: 探究葡萄籽油对四氯化碳（CCl₄）诱导小鼠急性肝损伤的保护作用。利用CCl₄建立急性肝损伤模型，通过测定小鼠体重、食物和水摄入量，检测血清和肝组织相关指标，观察小鼠肝组织形态学变化，以评价葡萄籽油对小鼠急性肝损伤的保护作用。结果表明：与CCl₄组相比，中、高剂量（10、20 mL/kg·d）葡萄籽油处理能有效降低肝损伤小鼠血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）、乳酸脱氢酶（LDH）活性以及总胆固醇（TC）和甘油三酯（TG）含量（P<0.05），显著提高肝组织中谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）活力（P<0.05），并降低丙二醛（MDA）含量（P<0.05），明显改善病理损伤程度。综上所述，葡萄籽油对CCl₄诱导小鼠急性肝损伤具有明显的保护作用，且剂量越高，保护作用越强。

Abstract: The hepatoprotective effects of grape seed oil (GSO) against carbon tetrachloride (CCl₄) induced acute liver injury in mice was investigated. The model of acute liver injury was induced by intraperitoneal injection of CCl₄. The body weight and food and water intake were measured, the relevant indexes of serum and liver tissue were detected, and the pathological changes of liver tissues in mice were observed. The results showed that compared mice-treated with CCl₄, the mice were oral administered middle dose (10 mL/kg·d) of GSO (MGSO) and high dose (20 mL/kg·d) of GSO (HGSO) could significantly reduce the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and the levels of total cholesterol (TC), triglyceride (TG) (P<0.05) in serum, could significantly increase the activities of glutathione (GSH), superoxide dismutase (SOD) and reduce concentrations of malondialdehyde (MDA) (P<0.05), and could significantly improve the pathological changes of liver tissue. Therefore, GSO had significant hepatoprotective effects against CCl₄ induced acute liver injury in mice, and the hepatoprotective effects was stronger with the increasing dose．