Abstract: The purpose of this experiment was to study the stability and antioxidant activity of Torreya grandis hydrosols in vivo and in vitro. The work aims to study the stability, volatile composition and antioxidant activity of Torreya grandis hydrosols. The stability of untreated and filter-treated hydrosols was evaluated by LUMi Sizer stability analyzer, and the volatile components of hydrosols were analyzed by gas chromatography-mass spectrometry (GC-MS). In addition, the antioxidant activity in vitro and in vivo was evaluated by free radical scavenging experiments and C.elegans tests respectively. The results showed that the filter-treated hydrosols revealed better stability as compared to untreated hydrosols, with the instability coefficient close to 0 (0.007±0.003). GC-MS demonstrated that a total of 49 volatile components were identified for the hydrosols, including 21 alcohols (41.82%), 7 terpenoids (5.27%), 5 phenols, 5 hydrocarbons, 3 aldehydes and some other types of volatile compounds. DPPH and ABTS free radical scavenging experiments demonstrated that T. grandis hydrosols revealed good antioxidant activity with radical scavenging rate of 67.3%±1.2% and 84.2%±3.7%, respectively. Furthermore, the hydrosols revealed significant antioxidant performance in C.elegans and prolong the life span of C.elegans (P<0.05). These results may help in developing new antioxidant and anti-aging ingredients for food and cosmetic products use.