马齿苋降尿酸及肾脏保护作用的研究

吕有为; 潘昱辰; 孙铭键; 姚航; 王曼力; 杜培革; 安丽萍

doi:10.13386/j.issn1002-0306.2021040074

马齿苋降尿酸及肾脏保护作用的研究

Effect of Portulaca oleracea L. on Lowering Uric Acid and Protecting Kidney

摘要

摘要: 目的：研究马齿苋降尿酸和肾脏保护作用，为临床研究提供参考。方法：将雄性ICR小鼠随机分为5组，空白组（CON）、模型组（HUA）、别嘌呤醇阳性药对照组（ALLOP，0.02 g/kg/d）、马齿苋高剂量组（PO-H，1.48 g/kg/d）、马齿苋低剂量组（PO-L，0.5 g/kg/d）。每组按相应给药剂量连续灌胃21 d，第7 d开始，除空白组外，每组腹腔注射次黄嘌呤（HX，0.3 g/kg）联合氧嗪酸钾（OAPS，0.3 g/kg）以建立高尿酸血症模型。观察小鼠血清尿酸值（SUA）、丙二醛（MDA）水平；收集肝脏检测黄嘌呤氧化酶（XOD）、超氧化物歧化酶（SOD）水平；收集肾脏检测谷胱甘肽（GSH）、谷胱甘肽过氧化酶（GSH-PX）水平；实验结束前一天收集24 h尿液检测尿尿酸（UUA）、尿肌酐（UCr）水平；对小鼠肾脏HE染色进行病理学观察。结果：与CON组比较，HUA组极显著（P<0.01）升高MDA、SUA、XOD水平，极显著（P<0.01）降低SOD、GSH、GSH-PX、UUA、UCr水平，说明高尿酸血症建模成功。与HUA组比较，PO-H组可极显著（P<0.01）降低SUA、MDA、XOD水平，极显著（P<0.01）升高SOD、GSH、GSH-PX、UCr水平；PO-L组可显著（P<0.05或P<0.01）降低SUA、MDA、XOD水平，显著（P<0.05或P<0.01）升高GSH、GSH-PX、UCr水平；HE染色结果显示PO可明显改善HUA小鼠肾组织的病理变化。结论：马齿苋提取物对高尿酸血症小鼠具有一定的降尿酸和肾保护功能。

Abstract: Objective: To study the uric acid-lowering and renal protective effects of Portulaca oleracea to provide reference for clinical research. Methods: Male ICR mice were randomly divided into 5 groups. Blank group (CON), model group (HUA), allopurinol positive drug control group (ALLOP, 0.02 g/kg/d), purslane high-dose group (PO-H, 1.48 g/kg/d), Portulaca low-dose group (PO-L, 0.5 g/kg/d). Each group was given the corresponding dose for 21 d continuously, starting from the 7th day, except for the blank group, each group was intraperitoneally injected with hypoxanthine (HX, 0.3 g/kg) combined with potassium oxazine (OAPS, 0.3 g/kg) to establish a hyperuricemia model. Observe serum uric acid (SUA) and malondialdehyde (MDA) levels in mice, collect liver to detect xanthine oxidase (XOD) and superoxide dismutase (SOD) levels, collect kidney to detect glutathione (GSH), glutathione peroxidase (GSH-PX) level. 24 hours urine was collected the day before the end of the experiment to detect urine uric acid (UUA) and urine creatinine (UCr) levels, pathological observation of mouse kidney HE staining was performed. Results: Compared with the CON group, the HUA group significantly (P<0.01) increased the levels of MDA, SUA, and XOD, and significantly (P<0.01) decreased the levels of SOD, GSH, GSH-PX, UUA, and UCr, indicating that hyperuricemia was a cause of hyperuricemia. The model was successful. Compared with HUA group, PO-H group could significantly (P<0.01) reduce SUA, MDA, XOD (levels, significantly (P<0.01), increase SOD, GSH, GSH-PX, UCr levels. PO-L group could significantly (P<0.05 or P<0.01) reduce the levels of SUA, MDA, XOD, and significantly (P<0.05 or P<0.01) increase the levels of GSH, GSH-PX, UCr. HE staining results showed that PO could significantly improve the kidneys of HUA mice tissue pathological changes. Conclusion: Portulaca oleracea extract would have certain uric acid-lowering and renal protective functions on hyperuricemia mice.