共轭亚油酸对高脂饲料诱导肥胖大鼠脂质蓄积和肠道菌群的影响

李彤; 黄芳; 陈杰东; 李文榜; 林岱; 郭福川

doi:10.13386/j.issn1002-0306.2021040049

共轭亚油酸对高脂饲料诱导肥胖大鼠脂质蓄积和肠道菌群的影响

Effects of Conjugated Linoleic Acid on Lipid Accumulation and Intestinal Microbiota in Obese Rats Induced by High-fat Diet

摘要

摘要: 目的：以高脂饲料诱导建立肥胖大鼠模型，探讨共轭亚油酸（CLA）干预对肥胖大鼠脂质蓄积和肠道菌群的影响。方法：将40只SPF级雄性SD大鼠按体重随机分为四组，每组10只，除对照组（ND组）以普通饲料喂养外，其余组均以高脂饲料喂养。其中ND组灌胃花生油1 g/kg；高脂模型组（HD组）灌胃花生油1 g/kg；高脂共轭亚油酸低剂量组（LC组）灌胃0.2%共轭亚油酸1 g/kg；高脂共轭亚油酸高剂量组（HC组）灌胃2%共轭亚油酸1 g/kg，干预9周。于实验末采集大鼠粪便后提取粪便中细菌DNA后进行16S rDNA测序，之后麻醉采血后处死，取肝脏、肾周脂肪和睾周脂肪组织，于−80 ℃保存。结果：HD组大鼠体重、内脏脂肪、血清和肝脏甘油三酯和总胆固醇显著高于ND组（P<0.05），高剂量共轭亚油酸干预可显著降低大鼠体重、血清甘油三酯和总胆固醇及肝脏总胆固醇（P<0.05）；高脂饲料诱导可使大鼠肠道菌群属水平中Eubacterium_coprostanoligenes_group（真杆菌属）、Psychrobacter（嗜冷杆菌属）、Corynebacterium_1（棒状杆菌_1）、Staphylococcus（葡萄球菌属）的相对丰度增加；高剂量CLA干预可改变肥胖大鼠的肠道菌群的组成，如在属水平上显著增加了Bifidobacterium（双歧杆菌属）、Butyrivibrio（丁酸弧菌属）、Ruminococcaceae_UCG-014（瘤胃球菌科_UCG-014）等的相对丰度（P<0.05），减少了Staphylococcus（葡萄球菌属）、Corynebacterium_1（棒状杆菌_1）等的相对丰度（P<0.05）。结论：共轭亚油酸可降低高脂饲料诱导肥胖大鼠的体重和脂质蓄积，其作用可能与调节大鼠肠道菌群组成有关。

Abstract: Objective: To investigate the effects of conjugated linoleic acid (CLA) on lipid accumulation and intestinal microbiota in obese rats. Methods: Forty SPF male SD rats were randomly divided into four groups according to body weight, 10 rats in each group. The ND group was fed with ordinary diet, and the other groups were fed with high-fat diet. Among them, the ND group was intragastrically administered with peanut oil 1 g/kg, the model group (HD group) was intragastrically administered with peanut oil 1 g/kg, the low-dose group (LC group) was intragastrically administered with 0.2% conjugated linoleic acid 1 g/kg, and the high-dose group (HC group) was intragastrically administered with 2% conjugated linoleic acid 1 g/kg for 9 weeks of intervention. Bacterial DNA was extracted from feces of mice after collection of feces at the end of the experiment followed by 16S rDNA sequencing, after which blood was collected and sacrificed under anesthesia, and organ and visceral fat were taken and stored at −80 ℃. Results: The body weight, visceral fat, serum and liver triglycerides and total cholesterol of rats in the HD group were significantly higher than those in the ND group (P<0.05), and high-dose CLA intervention could significantly reduce the body weight, serum triglycerides, total cholesterol and liver total cholesterol of rats (P<0.05). High-fat diet could increase the relative abundance of Eubacterium_coprostanoligenes_group, Psychrobacter, Corynebacterium_1 and Staphylococcus in the intestinal microbiota level of rats. High-dose CLA could change the composition of intestinal microbiota in obese rats, such as significantly increasing the relative abundance of Bifidobacterium, Butyrivibrio and Ruminococcaceae_UCG-014 at the genus level (P<0.05) and reducing the relative abundance of Staphylococcus and Corynebacterium_1 (P<0.05). Conclusion: Conjugated linoleic acid could reduce body weight and lipid accumulation of obese rats induced by high fat diet, which might be related to the regulation of intestinal microbiotal diversity.