瓶尔小草不同溶剂提取物的体外生物活性

曾永芳; 廖华珍; 许海棠; 王聪; 韦贻春

doi:10.13386/j.issn1002-0306.2021020167

瓶尔小草不同溶剂提取物的体外生物活性

Study on Biological Activity of Different Solvent Extracts from Ophiolossum vulgatum L. in Vitro

摘要

摘要: 比较瓶尔小草不同溶剂（氯仿、乙酸乙酯、正丁醇、乙醇）提取物的抗氧化性、抑菌性和抗癌细胞增殖作用。以抗坏血酸为对照，通过测定对3种自由基的清除作用来评价瓶尔小草提取物的抗氧化活性；以微量稀释法研究了4种提取物对几种常见致病菌的抑菌活性；同时还采用四甲基偶氮唑蓝（methyl thiazolyl tetrazolium, MTT）法研究了瓶尔小草提取物对SKOV3卵巢癌细胞的抗增殖作用。结果表明：瓶尔小草各提取物都有一定的抗氧化、抑菌和抗增殖作用。其中正丁醇提取物的活性最强，其对DPPH自由基的清除效果最佳，IC₅₀为0.114 mg/mL；对乙型溶血性链球菌、铜绿假单胞菌和白色念珠球菌有明显的抑制作用，MIC均为0.313 mg/mL；对SKOV3卵巢癌细胞的抑制作用最强，IC₅₀为114.5 μg/mL。瓶尔小草正丁醇提取物可望进一步开发用于药品和功能食品领域。

Abstract: The antioxidant, antibacterial and antiproliferative activities of different solvents (chloroform, ethyl acetate, n-butanol, ethanol) extracts from Ophiolossum vulgatum L. were compared. The antioxidant activity of four extracts was evaluated by determining the removal effect of three free radicals, with ascorbic acid used as control. The antimicrobial activity of the four extracts against several common pathogenic bacteria was determined by microdilution assay. The antiproliferative activities on SKOV3 cells were detected by MTT assay. The results showed that all the extracts had certain antioxidant, antibacterial and antiproliferative activities, especially n-butanol extract exhibited the strongest activity among them. The scavenging effect of n-butanol extract was the best for DPPH free radicals, with IC₅₀ value of 0.114 mg/mL, and it had obvious inhibitory effect on beta hemolytic Streptococcus, Pseudomonas aeruginosa and Monilia albican, with MIC value of 0.313 mg/mL, and had the strongest inhibitory effect on SKOV3 ovarian cancer cells, with IC₅₀value of 114.5 μg/mL. The n-butanol extract from Ophiolossum vulgatum L. showed the potential to be further studied and used in the fields of drugs and functional foods.