驴骨胶原蛋白成分分析及促进皮肤修复活性的研究

樊雨梅; 史传超; 帖航; 张筱梒; 赵海晴; 廖峰

doi:10.13386/j.issn1002-0306.2021010211

驴骨胶原蛋白成分分析及促进皮肤修复活性的研究

Study on the Nutritional Ingredients and the Effect on the Repair of Damaged Skin Barrier Function of Donkey Bone Collagen

摘要

摘要: 为从分子水平探究驴骨胶原蛋白的功效物质基础和驴骨胶原蛋白对受损皮肤屏障的修复作用，本实验采用超高效液相色谱综合高分辨率轨道分析质谱（UPLC-Orbitrap MS）技术定性分析驴骨胶原蛋白的有效成分，用5-溴脱氧尿嘧啶核苷（5-bromodeoxyuridine, BrdU）渗入法和细胞划痕实验检测驴骨胶原蛋白对角质形成细胞增殖和迁移的影响，以探究其对皮肤的修复作用。结果表明：驴骨胶原蛋白的蛋白含量为57.5%，具有典型的胶原氨基酸组成。驴骨胶原蛋白含有较为丰富的氨基酸、维生素、糖类和脂质等活性成分。0.15 g/mL的驴骨胶原蛋白促进人表皮角质形成细胞（HaCaT细胞）增殖和迁移的效果与5%胎牛血清类似，显著优于空白对照组（P<0.05）。最终推断驴骨胶原蛋白能够加速表皮屏障修复，其机制可能与促进角质形成细胞增殖和迁移有关。

Abstract: In order to explore the functional components of donkey bone collagen and the repair effect of donkey bone collagen on the damaged skin barrier. The functional composition of donkey bone collagen was analyzed by UPLC-Orbitrap MS technique, the effects of donkey bone collagen on the proliferation of HaCaT cells were detected by Brdu method, and the effects on the migration of HaCaT cells were detected by scratch method. The results showed that the protein content of donkey bone collagen was 57.5%, which had a typical collagen amino acid composition. The donkey bone collagen contained abundant functional ingredients such as amino acids, vitamins, sugar and lipids. Donkey bone collagen could significantly promote the proliferation and migration of HaCaT cells, and the effect of 0.15 g/mL donkey bone collagen in promoting the proliferation and migration of HaCaT cells was similar to that of 5% fetal bovine serum, which was significantly better than the blank control group (P<0.05). It is inferred that donkey bone collagen could accelerate the repair of skin barrier, and its mechanism might be related to promoting keratinocytes proliferation and migration.