Abstract: Objective: To investigate the renal-protective functions of total glucosides of paeony(TGP) on hyperuricemic renal damage(HN) rats. Methods: Fifty male SD rats were randomly divided into 5 groups: Normal group, model group, TGP low and high dose groups(100 and 300 mg·kg⁻¹·bw⁻¹), and allopurinol group(27.0 mg·kg⁻¹·bw⁻¹), with 10 rats in each group. At 7:00 a.m. and 20:00 p.m. every day, the model group, TGP low-dose group, TGP high-dose group and allopurinol group were given adenine 100 mg/kg+oteracil potassium 1500 mg/kg suspension respectively to make HN model. TGP and allopurinol were intragastrically administered at 12:00, the normal group and model group were given the same amount of normal saline every day. The observation time was 5 weeks. The body weight, serum uric acid, creatinine, urea nitrogen and other indicators of rats in each group were detected every two weeks. After 5 weeks, the ratio of bilateral kidney weight to body weight and the pathological section of kidney were observed. Results: At the 5th week, compared with the model group, TGP high-dose group could significantly reduce the renal weight(P<0.01), renal body ratio(P<0.01), serum uric acid(P<0.01), urea nitrogen(P<0.05) and creatinine(P<0.01) levels of HN model, alleviate the proliferation of glomerular mesangial cells, alleviate the expansion of renal tubules, and reduce tubulointerstitial injury and renal inflammatory cell infiltration of HN model. Conclusion: TGP had a protective effect on the kidney of HN rats.