高效液相色谱法测定鸡蛋、鸡肉中五种类胡萝卜素物质

兰韬; 闫娜; 裴栋; 刘佳雯; 于聪聪; 初侨; 李笃信

doi:10.13386/j.issn1002-0306.2021010109

高效液相色谱法测定鸡蛋、鸡肉中五种类胡萝卜素物质

Determination of Five Carotenoids in Eggs and Chicken by HPLC

摘要

摘要: 本文研究了鸡蛋、鸡肉中虾青素、叶黄素、斑蝥黄、玉米黄素、β-胡萝卜素等5种类胡萝卜素同时检测的HPLC方法。结果表明，5种类胡萝卜素在0.5~100 μg/mL线性范围内有较好的线性关系，决定系数R²均大于0.996，方法的检出限均在0.10~0.20 mg/kg之间，定量限在0.33~0.66 mg/kg之间。在1、10、20 mg/kg的加标水平下，鸡蛋和鸡肉样品的加标回收率分别在71.6%~112.9%和88.6%~119.7%之间，RSD均≤7.2%。该方法快速简单、准确度高、重复性好，适用于鸡蛋、鸡肉等复杂基质样品中5种类胡萝卜素类物质检测的要求，对于鸡蛋、鸡肉的市场监管具有重要意义。

Abstract: A HPLC method for simultaneous determination of astaxanthin, lutein, cantharidin, zeaxanthin and β-carotene in eggs and chicken was developed. The results showed a good linear relationship in the range of 0.5~100 μg/mL for five carotenes, and the coefficients of determination were all above 0.996. The limits of detection of the method were between 0.10 and 0.20 mg/kg, with the limits of quantitation were between 0.33 and 0.66 mg/kg. At the spiked levels of 1, 10 and 20 mg/kg, the recoveries of eggs and chicken samples were 71.6%~112.9% and 88.6%~119.7%, respectively, with RSD≤7.2%. The method is rapid, simple, accurate and reproducible. It is suitable for the detection of five kinds of carotenoids in complex matrix samples such as eggs and chicken. It is of great significance for the market supervision of eggs and chicken.