Abstract: Flaxseed oil was prepared from 40 kinds of flaxseed of different region in Qinghai by soxhlet extraction. And the volatile profiles were analyzed by headspace sampling combined with GC-MS method. In order to obtain the best solid phase microextraction conditions and GC-MS condition, the stirring rate, extraction temperature, equilibrium time, extraction time, desorption time and heating program were optimized. The results indicated that when using DVB/CAR/PDMS fiber, the optimum extraction conditions of the volatile compounds were: 20 min equilibrium time, 40 min adsorption time at 80 ℃ , stirring intensity of 400 r/min, and 5 min desorption time. Under this extraction condition, a total of 58 volatile components of 40 kinds of flaxseeds oil in Qinghai could be detected, including 12 aldehydes, 8 acids, 9 alcohols, 2 ketones, 5 esters, 13 hydrocarbon compounds, 3 heterocycles and 6 other types, which achieved a good chromatographic separation. According to cluster analysis, aldehydes were the main volatile components that distinguished flaxseed oil samples, which would provide a theoretical basis for the quality identification and development of flaxseed oil.