负载茶多酚的花生分离蛋白-聚乳酸纳米纤维膜的制备及抗菌性能研究

姚飞; 陈复生; 郝明飞

doi:10.13386/j.issn1002-0306.2021010033

负载茶多酚的花生分离蛋白-聚乳酸纳米纤维膜的制备及抗菌性能研究

Preparation and Antibacterial Properties of Peanut Protein Isolate-Polylactic Acid Nanofiber Membrane Loaded with Tea Polyphenols

摘要

摘要: 利用静电纺丝技术制备负载茶多酚（TP）的花生分离蛋白（PPI）-聚乳酸（PLLA）复合纳米纤维薄膜，并研究其微观形貌、红外光谱结构、热稳定性、疏水性、晶体结构和抑菌性能等。傅里叶红外光谱和 X 射线衍射结果显示，复合纳米纤维成功包埋了TP，实验添加的TP对PPI/PLLA纳米纤维形态分布影响不大，其平均直径从（185±57）nm增加到（222±72）nm，而疏水性和热稳定性有所降低，但TP/PPI/PLLA复合纳米纤维膜提高了TP的热稳定性。抑菌结果表明：TP/PPI/PLLA三元复合纳米纤维膜对大肠杆菌和金黄色葡萄球菌的抑菌圈宽度分别从（0.75±0.03）cm和（1.49±0.08）cm增加到（1.10±0.02）cm和（1.82±0.10）cm，且对金黄色葡萄球菌的抑菌效果大于大肠杆菌的抑菌效果。由此研究表明，TP/PPI/PLLA三元复合纳米纤维膜具有良好的潜在应用价值。

Abstract: Electrospinning technology was used to prepare peanut protein isolate (PPI)-polylactic acid (PLLA) composite nanofiber film loaded with tea polyphenols (TP), and to study its microscopic morphology, infrared spectrum structure, thermal stability, hydrophobicity, and crystals structure and antibacterial properties, etc. Fourier infrared spectroscopy and X-ray diffraction results showed that the composite nanofibers had successfully embedded TP. The experimentally added TP had little effect on the morphological distribution of PPI/PLLA nanofibers. The average diameter increased from (185±57) nm to (222±72) nm. The hydrophobicity and thermal stability were reduced, but the TP/PPI/PLLA composite nanofiber membrane improved the thermal stability of TP. The antibacterial results showed that the width of the inhibition zone of TP/PPI/PLLA ternary composite nanofiber membrane against Escherichia coli and Staphylococcus aureus increased from (0.75±0.03) and (1.49±0.08) cm to (1.10±0.02) and (1.82±0.10) cm, respectively. And the antibacterial effect of Staphylococcus aureus was greater than that of Escherichia coli. This study shows that the TP/PPI/PLLA ternary composite nanofiber membrane has good potential application value.