阿勒泰脂臀羊臀脂分提产物对小鼠肝脏脂质水平及氧化应激的影响

徐艳丽; 朱明睿; 孙佳宁; 张莉; 马玉洁; 王子荣

doi:10.13386/j.issn1002-0306.20210016

阿勒泰脂臀羊臀脂分提产物对小鼠肝脏脂质水平及氧化应激的影响

Effects of Altay Sheep Buttocks Fat Extract on Liver Lipid and Oxidative Stress in Mice

摘要

摘要: 为探讨阿勒泰脂臀羊臀脂及其分提产物对小鼠肝脏脂质水平和氧化应激的影响，将84只昆明小鼠随机分为20 d和40 d两个批次，每批7组（n = 6）：空白组（基础饲料喂养）、菜籽油组、原脂组、2 ℃固脂、12 ℃固脂、22 ℃固脂、32 ℃固脂组，实验油脂组以基础饲料喂养+0.15 mL/10 g的油脂灌服小鼠，测定小鼠肝脏指数，进行肝脏组织病理学观察，按照试剂盒说明书测定肝脏脂质水平及氧化应激指标。结果表明：阿勒泰脂臀羊臀脂及其分提产物对小鼠肝脏指数无显著影响（P>0.05），肝脏组织无病理性变化；与菜籽油相比，分提产物可有效降低小鼠肝脏脂质水平，其中2 ℃固脂效果更显著（P<0.05）。40 d时，菜籽油组丙二醛（Malondialdehyde，MDA）含量显著高于各组（P<0.05）；32 ℃固脂超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化氢酶（Catalase，CAT）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）活性及总抗氧化能力（Total antioxidation，T-AOC）均显著升高（P<0.05），而MDA含量显著下降（P<0.05）。随着摄入时间的增长，菜籽油组肝指数显著升高8.21%，原脂组总胆固醇（Total cholesterol，TC）水平显著下降，高密度脂蛋白胆固醇（High density lipoprotein cholesterol，HDL-C）水平显著上升，32 ℃固脂组MDA含量显著降低，SOD活性显著升高30.85%。阿勒泰脂臀羊臀脂的不同分提产物对小鼠肝脏脂质水平、氧化应激产生不同的影响。相比菜籽油，未分提的原脂更有益于人体健康，羊臀脂分提产物中2 ℃固脂可有效控制小鼠肝脏脂质上升，32 ℃固脂提升抗氧化酶活性、减少氧化损伤的效果更优。

Abstract: To investigate the effects of Altay lipid and its extract on liver lipid level and oxidative stress in mice, 84 Kunming mice were randomly divided into two batches of 20 days and 40 days, 7 groups each (n=6): blank group (basic feed feeding), rapeseed oil group, Altay sheep buttocks oil group, 2 ℃ solid oil group, 12 ℃ solid oil group, 22 ℃ solid oil group, 32 ℃ solid oil group, experimental oil group fed 0.15 mL/10 g of basic feed to mice, the liver index of mice was measured, pathology were observed, liver lipid level and oxidative stress index were determined according to the kit specification. The results showed that there was no significant effect on liver index of mice with Altay sheep buttocks fat and its extract (P>0.05), no pathological changes in liver tissue, compared with rapeseed oil, the Altay sheep buttocks fat extracts could effectively reduce the liver lipid level of mice, and the effect of 2 ℃ solid oil group was more significant (P<0.05). At 40 days, the MDA content of the rapeseed oil group was significantly higher than that of the other groups (P<0.05); Compared with the blank group, 32 ℃ solid fat group SOD, CAT, GSH-Px enzyme activities and T-AOC were significantly increased (P<0.05), while the MDA content was significantly decreased (P<0.05). With the increase of intake time, liver index of rapeseed oil group increased by 8.21% significantly, the TC level of the Altay sheep buttocks oil group decreased significantly, and the HDL-C level increased significantly, the MDA content of 32 ℃ solid fat group decreased significantly and the SOD activity increased 30.85% significantly. Different Altay sheep buttocks fat extracts has different effects on the liver lipid levels and oxidative stress in mice. Compared with rapeseed oil, Altay sheep buttocks oil is more beneficial to human health. The 2 ℃ solid oil in the Altay sheep buttocks fat extracts can effectively control the increase of liver lipids in mouse, and the 32 ℃ solid oil enhances antioxidant enzyme activity and reduces oxidation damage effect is better.