Abstract: To reveal the difference and diversity of fungal community structure on the surface of grape and rhizosphere soil from the different areas in Tibet, high-throughput sequencing technology was used to analyze the fungal diversity and community structure in grape and rhizosphere soil samples collected from Mangkang and Linzhi in Tibet. The results showed that the rhizosphere soil in Mangkang had a highest diversity of fungi, while the lowest on Linzhi grape surface. Ascomycota was the dominant phylum at phyla level, accounting for 76.06%~99.60%. At genus level, Cladosporium and Hanseniaspora were the dominant genus in LG and MG, respectively, accounting for 64.43% and 31.59%; while the dominant genus of LS and MS were Fusarium and Mortierella, respectively, accounting for 50.02% and 20.16%. Principal component analysis showed that the fungal community structure was significantly different in different samples, and the fungal community structure of grape epidermis and rhizosphere soil was similar in the different area. Redundancy analysis (RDA) showed that TK, pH, TN, TP and AN had a great influence on fungal community, but the effect of EC and SOM was not obvious. In conclusion, the surface of grape and rhizosphere soil microbial diversity from the different areas in Tibet were very rich, the fungal community structure was significantly similar in two areas. The study of grape and rhizosphere microbial diversity in Tibet could provide a theoretical basis for the utilization and development of characteristic microorganisms in this area.