蓝莓酵素的体外抗氧化及对秀丽隐杆线虫的氧化应激保护作用

王高坚; 王珍珍; 李嘉嘉; 范昊安; 沙如意; 毛建卫

doi:10.13386/j.issn1002-0306.2020110157

蓝莓酵素的体外抗氧化及对秀丽隐杆线虫的氧化应激保护作用

Antioxidant Activity in Vitro and Promoting Resistance to Oxidative Stress in Caenorhabditis elegans of Blueberry Jiaosu

摘要

摘要: 以大兴安岭野生蓝莓为原料，发酵制备蓝莓酵素，研究其发酵过程中总花青素、总酚和总黄酮含量变化，以及体外抗氧化能力变化情况，并以秀丽隐杆线虫为模型，初步探究其抗氧化的相关机制。结果表明：在发酵过程中，总花青素含量呈先上升后下降的变化趋势，于发酵第50 d时，达到了0.52 mg/mL，总酚和总黄酮含量总体呈现上升趋势，均于第300 d时达到最大值2.81、3.17 mg/mL；1, 1-二苯基-2-苦苯肼（1, 1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除能力、还原力、氧自由基吸收能力（oxygen-radical absorbance capacity，ORAC）均呈现先快速增长后趋于平缓的变化趋势。蓝莓酵素能够延长秀丽隐杆线虫寿命，增强其在双氧水刺激下的氧化应激能力，提高超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）等抗氧化酶的活力，并且降低线虫体内活性氧和丙二醛含量，该结果表明蓝莓酵素可能是通过提高抗氧化酶活力来减缓秀丽隐杆线虫的氧化损伤。

Abstract: Blueberry Jiaosu fermented by using wild blueberries (Vaccinium uliginosum L) was used as raw materials. The changes of the total anthocyanin, phenolic, flavonoid contents, and antioxidant capacity in vitro during fermentation were detected. Caenorhabditis elegans were used to determine the antioxidant activity in vivo, preliminarily explored the related mechanism of antioxidant of blueberry Jiaosu. The results showed that the total anthocyanin content increased firstly and then decreased, reaching 0.52 mg/mL on the 50th day. The total phenolic and flavonoid contents kept increasing and reached their maximum level (2.81 mg/mL, 3.17 mg/mL, respectively) on the 300th day. In C.elegans, the results showed that blueberry Jiaosu could prolong the lifespan of C.elegans, enhance its oxidative stress ability under the stimulation of hydrogen peroxide, increase the activities of superoxide dismutase, catalase and glutathione peroxidase, and reduce the content of reactive oxygen species and malondialdehyde. These indicate that blueberry Jiaosu may increase the activity of antioxidant enzymes to reduce the oxidative damage of Caenorhabditis elegans.