Abstract: Infants, especially newborns, have poor digestion and immune function and immature intestinal development, leading to poor intestinal tolerance and difficulty in bacterial colonization, and highly sensitivity to necrotizing enterocolitis and infectious diarrhea. The benefits of breast feeding for infant intestinal health depend on a special oligosaccharide, and 2'-fucosyllactose (2'-FL) is the higher content of oligosaccharides. This paper describes the safety of 2'-FL, regulation of intestinal flora and immune system, and its protective effect on necrotizing enterocolitis and infectious diarrhea in infants.