响应面优化超声波辅助酶法提取油莎豆ACE抑制肽的工艺

殷海洋; 刘振春; 张世康; 安琪

doi:10.13386/j.issn1002-0306.2020100130

响应面优化超声波辅助酶法提取油莎豆ACE抑制肽的工艺

Optimization of Ultrasonic-assisted Enzymatic Extraction of ACE Inhibitory Peptides from Cyperus esculentus by Response Surface Method

摘要

摘要: 本文在单因素实验的基础上用响应面法优化了超声辅助酶提取油莎豆ACE（angiotensin converting enzyme）抑制肽工艺，并通过对血管紧张素转化酶的抑制实验选取了最佳辅助酶。结果表明，底物浓度3%、超声处理20 min、酶解温度45 ℃、加酶量5000 U/g、超声功率180 W、酶解3 h是超声波辅助酶法提取油莎豆ACE抑制肽的最佳工艺条件，最佳辅助酶-碱性蛋白酶，在此条件下ACE抑制率为74.16%。本研究为提取油莎豆ACE抑制肽提取了一定理论依据，为进一步研究油莎豆ACE抑制肽奠定了基础。

Abstract: In this paper, based on the single-factor experiment, the response surface method was used to optimize the ultrasonic-assisted enzyme extraction process of Cyperus esculentus ACE inhibitory peptide, and the best auxiliary enzyme was selected through the inhibition experiment of angiotensin converting enzyme. The results showed that the optimal process conditions for the ultrasonic-assisted enzymatic extraction of Cyperus esculentus ACE inhibitory peptides were: Substrate concentration 3%, ultrasonic treatment time 20 min, enzymolysis temperature 45 ℃, enzyme addition 5000 U/g, ultrasonic power 180 W, enzymolysis time 3 h, the best auxiliary enzyme-alkaline protease, under this condition, the ACE inhibition rate was 74.16%. This study would provide a theoretical basis for the extraction of Cyperus esculentus ACE inhibitory peptides, which laid a foundation for further research on Cyperus esculentus ACE inhibitory peptide.