亚油酸水合酶生物合成微生物源羟基不饱和脂肪酸的研究进展

彭舒悦; 梁暖意; 张延镇; 郭前婉; 王琪; 赵萌; Michael G.Gänzle

doi:10.13386/j.issn1002-0306.2020100119

亚油酸水合酶生物合成微生物源羟基不饱和脂肪酸的研究进展

Research Progress in Biosynthesis of Microbial Hydroxyl Unsaturated Fatty Acids by Linoleic Acid Hydratase

摘要

摘要: 羟基不饱和脂肪酸（Hydroxy unsaturated fatty acids, HUFA）是一类功能性脂肪酸，其生物合成法特异性强、对环境友好，是HUFA制备的主要方法之一。本文就两种较受关注的微生物源HUFA，即10-羟基-12-十八碳烯酸（10-hydroxy-12-octadecenoic acid，10-HOE）、13-羟基-9-十八碳烯酸（13-hydroxy-9-octadecenoic acid，13-HOE）进行了来源、功能特性及应用的介绍，归纳总结了用于合成10-HOE和13-HOE的亚油酸水合酶的研究进展；最后展望了HUFA功能特性和生物合成的研究方向，建议基于胶体界面化学理论来提高亚油酸水合酶合成HUFA的转化效率。

Abstract: Emerging studies have shown the important bioactivities of hydroxy unsaturated fatty acids(HUFA). The bioproduction of HUFAs is highly specific and environmentally friendly, which has become one of the main preparation methods of HUFA. The sources, functions and applications of two most studied microbial HUFAs, namely 10-hydroxy-12-octadecenoic acid(10-HOE) and 13-hydroxy-9-octadecenoic acid(13-HOE), are first discussed. Then, the research progress of linoleic acid hydratases(LHT), one type of microbial enzymes responsible for the synthesis of 10-HOE and 13-HOE, is reviewed. Finally, the further researches of the bioactivities and biosynthesis of HUFA are proposed, in which the bioconversion efficiency of HUFA with LHT can be improved based on the theory of colloid and interface chemistry.