鹿鞭醇提物对秀丽隐杆线虫衰老的影响

迟东泽; 何源; 刘芳芳; 张晶

doi:10.13386/j.issn1002-0306.2020090108

鹿鞭醇提物对秀丽隐杆线虫衰老的影响

Effect of Ethanol Extracts of Penis Cervi on Anti-aging in Caenorhabditis elegans

摘要

摘要: 目的：本文采用秀丽隐杆线虫衰老模型，研究了梅花鹿鞭醇提物（HC）和马鹿鞭醇提物（MC）的抗衰老活性差异及其作用机制，旨在为鹿鞭药材作为医药、保健品开发提供参考依据。方法：给予秀丽隐杆线虫鹿鞭提取物（0.5、1.0、2.0 mg/mL），测定了花鹿鞭和马鹿鞭醇提物对秀丽隐杆线虫寿命、吐咽能力、运动能力、生殖能力及应激抵御能力以及线虫体内的抗氧化酶活力和丙二醛（MDA）水平的影响，探讨了鹿鞭醇提物的抗衰老作用。结果：与空白组相比，2种鹿鞭在0.5、1.0 mg/mL时显著提高线虫寿命值（P<0.05），2.0 mg/mL时可极显著提高线虫的寿命值（P<0.01）且不影响生殖能力，显著提高线虫的吐咽、运动能力；对热应激和氧化应激具有显著的防御效果且降低ROS水平；在2.0 mg/mL水平，HC组的各项指标检测结果均显著强于MC。HC组线虫平均寿命为19.98±1.26 d，显著高于MC组的17.26±1.34 d；对热应激和氧化应激，HC组线虫的平均生存时间分别提高44.78%、41.04%，显著高于MC组25.71%、31.45%；运动测试结果显示，在第8 d，HC组运动A状态线虫提升29%，较MC高8.3%；HC使线虫ROS水平降低程度强于MC组；测定显示，HC与MC对线虫的生殖和吐咽能力影响无显著差异。结论：与空白组相比，2种鹿鞭醇提物均可抑制线虫体内ROS累积，增强对高温、百草枯急性氧化应激的抵抗能力、提高抗氧化酶活力，进而延长线虫寿命，具有抗衰老作用，而HC的活性强于MC。

Abstract: Objective: The differences in the anti-aging activities of alcohol extract of penis cervi of Cervus nippon(HC) and Cervus elaphus (MC) and their mechanisms of action were investigated using the Caenorhabditis elegans aging model. The purpose of the study, however, provide a reference for the development of penis cervi herbal medicine as a pharmaceutical and health care product. Methods: Extracts of penis cervi (0.5, 1.0, 2.0 mg/mL) were used to determine the lifespan, pharyngeal capacity, motility, reproductive capacity, and stress resistance of ethanol extracts of HC and MC on C.elegans, and the effects of penis cervi on antioxidant enzyme activity and malondialdehyde(MDA) levels in vivo were also included. In addition, the anti-aging effect of penis cerviethanol extracts was investigated. Results: Compared with the blank group, the two kinds of penis cervion significantly increased the life span of nematodes at 0.5 and 1.0 mg/mL (P<0.05), and significantly increased the life span of nematodes at 2.0 mg/mL (P<0.01), but did not affect the reproductive ability, significantly improved the swallowing and movement ability of nematodes; it had significant defense effect on heat stress and oxidative stress, and reduced ROS level; The results demonstrated that at the level of 2.0 mg/mL, the results of all parameters of HC were significantly stronger than those of MC. The mean lifespan of C.elegans in HC group was 19.98 ± 1.26 d, which was significantly higher than that of 17.26 ± 1.34 d in MC group. The mean survival time of C.elegans in the HC group increased by 44.78% and 41.04% for heat and oxidative stress, respectively, which was significantly higher than that of 25.71% and 31.45% in the MC group. Exercise test results indicated that at 8 d, HC group exercise A status C.elegans elevated 29%, which was 8.3% higher than MC. HC reduced ROS levels in C.elegans to a greater extent than in the MC group. The effects of HC and MC on the reproductive and pharyngeal abilities of C.elegans were not significantly different. Conclusion: Compared with the blank group, The results showed that the two ethanol extracts of penis cervi could inhibit the accumulation of ROS in C.elegans, enhance the resistance to high temperature and acute oxidative stress of paraquat, increase the activity of antioxidant enzymes, and consequently prolong the lifespan of C.elegans and have anti-aging effect, as well as the activity of HC was greater than that of MC.