基于黑磷纳米片的荧光适配体传感器检测雌激素17<i>β</i>-雌二醇

刘晓; 朱成龙; 庞月红; 冯永巍; 史学丽; 张毅

doi:10.13386/j.issn1002-0306.2020090046

基于黑磷纳米片的荧光适配体传感器检测雌激素17β-雌二醇

Fluorescence Aptasensor for 17β-Estradiol Determination Based on Black Phosphorus Nanosheets

摘要

摘要: 建立一种定量检测雌激素17β-雌二醇（E2）的荧光适配体传感器。以黑磷晶体为原料，利用超声辅助液相剥离技术制备了黑磷纳米片（BPNs）作为荧光受体，基于6-羧基荧光素标记的适配体（FAM-Apt）与BPNs间的荧光共振能量转移机制构建了荧光适配体传感器，对BPNs浓度、FAM-Apt浓度和反应时间进行优化，并对自来水和牛奶样品进行测定。结果表明，该BPNs具有独立的片层结构且粒径均匀；在最优条件下，即BPNs和FAM-Apt的浓度分别为10 μg/mL和7.5 nmol/L时，该传感器对E2检测的线性范围为1.5～60 ng/mL，检测限为1.0 ng/mL。自来水和牛奶样品中加标回收率分别为91.2%～104.8%和87.5%～104.3%；相对标准偏差（RSD）为4.99%～10.53%和4.48%～11.24%。该方法简便、灵敏，30 min即可完成检测，特异性强，能够实现对实际样品中E2的定量检测。

Abstract: A fluorescence aptasensor for quantitative detection of 17β-estradiol was constructed. Black phosphorus nanosheets (BPNs) were prepared by ultrasound-assisted aqueous phase exfoliation using black phosphorus crystal (BP) as fluorescence receptors. A 17β-estradiol fluorescence aptasensor was constructed between FAM labeled aptamers and BPNs based on the fluorescence resonance energy transfer (FRET). After optimization of BPNs concentration, FAM-Apt concentration and reaction time, samples of tap water and milk were determined. The results showed that the prepared BPNs had independent lamellar structure and uniform particle size.With the concentrations of BPNs and FAM-Apt were 10 μg/mL and 7.5 nmol/L respectively, the linear range of the sensor was 1.5~60 ng/mL and the detection limit was 1.0 ng/mL. The recoveries of tap water and milk were 91.2%~104.8% and 87.5%~104.3%. The relative standard deviation (RSD) were 4.99%~10.53% and 4.48%~11.24%, respectively. This method with simple, high sensitive and strong specificity could be completed within 30 min and could realize quantitative detection of E2 in actual samples.