Abstract: A new formula of sports beverage was developed by polyphenols of Acanthopanax sessiliflorus as raw materials. On the basis of single factor tests, response surface test was used to determine the best proportion of sports drink components. The exhaustive swimming time and related lactic acid biochemical were as indexes in different groups of mice, respectively. The optimum formula of sports beverage was obtained, when the polyphenols amount of Acanthopanax sessiliflorus was 10.12%, the amount of lemon juice was 16%, the amount of saccharose was 7.24%, the amount of sodium carboxymethylcellulose was 0.3%, and the amount of xanthan gum was 0.15%. The sensory evaluation score of the beverage under optimum process preparation was (88.6±1.7) score. The beverage solution presented an orange color, transparent, with the proper acidity and sweet, and had strong fragrance of lemon, without obvious precipitation or stratification.Compared with the blank control group, the sports beverage at middle and high doses could significantly prolong the swimming time in mice (P<0.01), enhance the LDH activity (P<0.05, P<0.01) and obviously decrease BLA content (P<0.05, P<0.01) in vivo. Therefore, the sports beverage of Acanthopanax sessiliflorus had better fatigue relieving effect.