Abstract: In this study, ICR male mice were divided into a control group (fed with ordinary diet) and a umi group (fed with umi diet), and analyzed the changes in body weight and serum physio-biochemical indices of the mice fed with Sphacelotheca sorghi (Link) Clint, and use high-throughput sequencing technology to analyze the diversity, community composition and community changes of intestinal microbes. The results of the study showed: Umi could inhibit the weight gain of mice, and could significantly reduce serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) content compared with the control group. The content of lipoprotein (HDL) increased significantly. Umi could also change the intestinal microbial communities of mice. Compared with the control group, the abundance of Actinobacteria and Firmicutes in the intestinal flora of mice in the Umi group increased, while the abundance of Bacteroidetes decreased. At the genus level, Umi could increase the abundance of short-chain fatty acid-producing bacteria such as Acinetobacter, Clostridium, Clostridium XlVa, and Ruminococcus in the intestinal flora of mice, and reduce the abundance of Barnesiella and Lactobacillus. At the species level, the abundance of intestinal microbe Muribaculum intestinale in the Umi group decreased significantly, while the abundance of Kineohrix alysoides increased significantly. In summary, Sphacelotheca sorghi (Link) Clint can effectively control the weight gain of mice, regulate the intestinal microbial communities of mice, and increase the abundance of beneficial bacteria.