猪宰后背最长肌中μ-calpain、pH和骨架蛋白变化与持水性的关系

杨波若; 李华健; 苏娅宁; 李霞; 瞿静; 陈韬

doi:10.13386/j.issn1002-0306.2020070347

猪宰后背最长肌中μ-calpain、pH和骨架蛋白变化与持水性的关系

Relationship Among Changes of μ-calpain, pH and Skeleton Protein and Water Holding Capacity of Post-mortem Pork

摘要

摘要: 为研究宰后猪肉中pH、μ-calpain、肌间线蛋白（desmin）和整联蛋白（integrin）变化与持水性的关系，将20条不同汁液流失的猪背最长肌样品按汁液流失率的高低，分成高汁液流失组（High drip loss；H组，drip loss≥5.01%，n=10）和低汁液流失组（Low drip loss；L组，drip loss≤4.00%，n=10）。分别于宰后45 min，3、9、12和24 h测定所选背最长肌pH、μ-calpain活性和desmin、integrin的表达量。结果表明：H组的汁液流失率极显著高于L组（P < 0.01），3～24 h的desmin表达量均高于L组（P < 0.05），45 min、12 h和24 h的μ-calpain活性低于L组（P < 0.05），integrin（45 min、3 h和12 h）的表达量和pH（45 min～12 h）均低于L组（P < 0.05）。desmin的表达量与汁液流失率呈正相关，而pH、μ-calpain活性和integrin的表达量与汁液流失率均呈负相关。pH、μ-calpain活性、desmin和integrin的表达量分别解释了汁液流失变异的78.1%、42.4%、70.3%和71.7%。上述结果表明：pH、μ-calpain、desmin和integrin都会对汁液流失产生影响；pH是对汁液流失率的影响最大的单变量。

Abstract: To investigate the relationship between pH, μ-calpain, desmin and integrin changes and water holding capacity in postmortem pork, 20 samples of longissimus dorsi with different drip loss were divided into High drip loss group(H-group drip loss≥5.01%, n = 10) and Low drip loss group (L-group drip loss≤4.00%, n = 10). pH, μ-calpain activity and expression levels of desmin and integrin were determined in 45 min, 3, 9, 12 and 24 h after slaughter. The results showed that the drip loss of H-group was significantly higher than L-group (P<0.01), desmin expression level (3～24 h) was higher than that of L group (P<0.05), the activity of μ-calpain (12 h and 24 h) was lower than that of L group (P<0.05), integrin expression level (45 min, 3 h and 12 h) and pH (45 min～12 h) was lower than that of L group. The expression of desmin was positively correlated with the drip loss, while the expression of integrin, the activity of μ-calpain and pH were negatively correlated with the driploss.pH, the express level of μ-calpain autolysis, desmin and integrin explained 78.1%, 42.4%, 70.3% and 71.7% of the variation in drip loss, respectively .The results showed that pH, μ-calpain, desmin and integrin all had an effect on drip loss, pH is the single variable that has the greatest influence on drip loss.