Abstract: Objective: To investigate the synergistic anti-tumor effect of Dihydromyricetin (DMY) and Myricitrin (MYT), which were the main active compounds in vine tea. Methods: The HepG2 cells were treated with DMY, MYT and DMY: MYT at the ratios of 1:4, 1:2, 1:1, 2:1, 4:1, 8:1, respectively. The activity of HepG2 cell was measured by MTT assay and fluorescence staining. The synergistic effect of DMY and MYT was analyzed by combination index (CI) and isobole. Results: DMY and MYT could inhibit the proliferation of HepG2 cells with IC₅₀ values of 56.46 and 95.01 μmol/L, respectively. When the mixed ratios of the two compounds were 1:4～8:1, their IC₅₀ values on the HepG2 cell proliferation were 40.90～73.00 μmol/L, and the CI values were 0.60～0.86. The mixed ratio of 1:1 showed the strongest synergistic effect, isobole analysis also indicated that there was a synergistic effect between two compounds. Conclusions: The combination of DMY and MYT could effectively promote the activity on inhibiting HepG2 cell proliferation, showing a synergistic effect, which would provide a basis for the further functional research and application of vine tea.