静乐黑枸杞花青素的纯化及其抗氧化特性

原红霞; 侯倩宜; 杜姗; 田欢; 李青山

doi:10.13386/j.issn1002-0306.2020070221

静乐黑枸杞花青素的纯化及其抗氧化特性

Purification and Antioxidant Properties of Anthocyanins from Jingle Lycium ruthenicum

摘要

摘要: 目的：探讨静乐黑枸杞花青素的纯化工艺及其抗氧化活性。方法：比较HPD100、D101、NKA、AB-8、HPD400等五种树脂对静乐黑枸杞花青素的吸附与解析性能，筛选最佳树脂，并优化其纯化条件；采用DPPH自由基、OH自由基和ABTS自由基法，比较黑枸杞样品纯化前后的抗氧化活性。结果：HPD100大孔树脂对于静乐黑枸杞花青素有良好的纯化性能，适宜的工艺条件为：静乐黑枸杞粗提液上样浓度为0.2 mg/mL（含生药量）、上样体积为49 mL、洗脱剂为75%的乙醇溶液、洗脱剂用量42 mL，在此条件下，纯化后花青素的纯度由2.38%提高至17.82%。静乐黑枸杞具有较好的抗氧化能力，其粗提液和纯化液清除DPPH·的IC₅₀ 值分别为0.208 和0.011 mg/mL；对ABTS⁺·清除能力的IC₅₀ 值分别为0.476 和0.064 mg/mL；纯化液清除·OH 的IC₅₀ 值为6.24 mg/mL。结论：大孔树脂吸附法分离纯化静乐黑枸杞花青素工艺合理，且纯化后抗氧化活性明显提高。

Abstract: Objective: To investigate the purification technology and antioxidation ability of Lycium ruthenicum anthocyanins from Jingle. Methods: Based on the adsorption properties of selected five macroporous resins (HPD100、D101、NKA、AB-8、HPD400) for Lycium ruthenicum anthocyanins from Jingle, the optimum purification resins were screened and the purification parameters were optimized. The antioxidant activity of crude and purified Lycium ruthenicum anthocyanins from Jingle were investigated by DPPH, hydroxyl and ABTS free radical scavenging assay. Results: HPD100 resin had good separation and purification effect. The optimum purification parameters were as follows: Sample concentration 0.2 mg/mL, sample volume 49 mL, elution volume 42 mL with 75% ethanol. After purification, the anthocyanins content was from 2.38% to17.82%. The crude and purified Lycium ruthenicum anthocyanins from Jingle showed good antioxidant activity, the IC₅₀ values of DPPH· scavenging capacity were 0.208 and 0.011 mg/mL respectively, the IC₅₀ values of ABTS⁺· scavenging capacity were 0.476 and 0.064 mg/mL respectively, the IC₅₀ value of purified Lycium ruthenicum anthocyanins for ·OH scavenging capacity was 6.24 mg/mL. Conclusion: The macroporous resin method for purifying Lycium ruthenicum anthocyanins has good purification effect. The antioxidant capacity of the purified Lycium ruthenicum anthocyanins was significantly improved compared with the crude extracts.