响应面法优化白首乌多糖超声辅助提取工艺及其结构表征

朱和权; 冯进; 李春阳; 吴承东; 张明; ChuonMony Roth

doi:10.13386/j.issn1002-0306.2020070135

响应面法优化白首乌多糖超声辅助提取工艺及其结构表征

Optimization of Ultrasonic-assisted Extraction Polysaccharide from Cynanchum auriculatum Royle ex Wight by Response Surface Methodology and Its Characterization

摘要

摘要: 为了提高白首乌多糖的得率，本文采用超声波辅助法提取白首乌中非淀粉多糖。在单因素实验基础上结合响应面法对超声波辅助法提取白首乌多糖工艺进行优化。结果表明，最优提取工艺参数为：液料比为22:1 mL/g、超声时间为44 min、超声功率为350 W及超声温度为52 ℃，在此条件下的多糖得率为1.35%±0.02%。该条件下提取的多糖的重均分子量集中在1.71×10⁷ 、1.15×10⁶、5.93×10⁴Da ，由岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖、核糖、半乳糖醛酸以及葡萄糖醛酸组成，含量分别为0.17%、1.66%、7.40%、10.51%、74.41%、0.79%、0.85%、0.36%、3.28%、0.57%，具有一般多糖的红外光谱吸收峰以及良好的溶解性, 为日后白首乌多糖的结构解析以及工业应用提供一定的参考。

Abstract: In order to improve the extraction yield of Cynanchum auriculatum Royle ex Wight, ultrasonic-assisted extraction was used to extract polysaccharide from Cynanchum auriculatum Royle ex Wight. The response surface method was used to optimize the ultrasonic-assisted extraction conditions of polysaccharide from Cynanchum auriculatum Royle ex Wight. The optimized ultrasonic-assisted extraction conditions of polysaccharide from Cynanchum auriculatum Royle ex Wight were the following: Solvent to material ratio 22: 1 mL/g, extraction time 44 min, ultrasonic power 350 W and temperature 52 ℃. Under these conditions, polysaccharide yield was 1.35%±0.02%. The average molecular weight of polysaccharides was mainly composed of 1.71×10⁷, 1.15×10⁶, 5.93×10⁴Da, and the polysaccharides mainly consisted of fucose, rhamnose, arabinose, galactose, galactose, xylose, mannose, ribose, galacturonic acid and glucuronic acid, with contents of 0.17%, 1.66%, 7.40%, 10.51%, 74.41%, 0.79%, 0.85%, 0.36%, 3.28%, 0.57%, respectively. FTIR spectrum showed characteristic absorptions of polysaccharide was observed in polysaccharides of Cynanchum auriculatum Royle ex Wight. The polysaccharides had good solubility. The study could provide some reference for structure analysis and industrial application of polysaccharides from Cynanchum Auriculatum Royle ex Wight.