Abstract: The interaction between food compounds is one of the core issues in the field of food science. In this work, various analytical techniques of polyphenol-protein interaction classified by direct and indirect way are reviewed, including UV-Vis absorption spectrometry, Fourier transform infrared spectroscopy, Raman spectroscopy, atomic force microscopy, isothermal titration calorimetry, molecular docking, molecular dynamic simulation and so on. Features and examples of these analytical techniques are summarized to provide technical support for the research of polyphenol-protein interaction, as well as the other food compounds interaction. Finally, this work puts forward the strategy and future work for the study of the polyphenol-protein interaction, and discusses the practical significance of such interaction studies in food processing .