Abstract: To explore the suitable thickness of packing material for decompression storage of Pleurotus eryngii, 0.03, 0.05, 0.07 mm low density polyethylene bags (LDPE) were vacuumed to 0.06 MPa and then refrigerated under 4 ℃ for 30 d to explore the physiological, biochemical indicators and quality effects of different thickness of packaging materials on Pleurotus eryngii under decompression storage conditions. The results showed that, by the end of storage, compared with the 0.03 and 0.05 mm treatment groups, the moisture content of the Pleurotus eryngii in the 0.07 mm low density polyethylene bag group was 80.42%, which could effectively reduce the water loss, inhibite the respiratory rate, slow down the decline of catalase (CAT) and superoxide dismutase (SOD) activities, inhibited the increase of malondialdehyde (MDA) content and polyphenol oxidase (PPO) activity, the smaller the effect on the texture characteristics and color quality of Pleurotus eryngii, such as hardness, cohesion, elasticity, and chewiness. Hence, the 0.07 mm low density polyethylene bag was the suitable packing material thickness for decompression storage of Pleurotus eryngii, and the preservation effect was better, which effectively delayed the post-maturing aging of Pleurotus eryngii and prolonged the preservation period of Pleurotus eryngii.