Abstract: Glyphosate and other strong polar pesticides are widely used in agriculture, and their ontology and metabolites are widely residual in food, soil, water and ecological environment. Because of their toxicity, the monitoring of residual glyphosate and other strong polar pesticides has attracted considerable attention. In this paper, the recent researches on the detection methods of strong polar pesticides are summarized, including the research status and applications of derivation-chromatography-mass spectrometry, non-derivation-liquid chromatography-mass spectrometry, ion chromatography and ion chromatography-tandem mass spectrometry, capillary electrophoresis, chromatography-ICP-MS or ICP-MS/MS combined with chromatography, rapid detection technology (enzyme-linked immunoadsorption method, immunosensor method, ion migration spectrum (IMS) method, molecular imprinting-chemical sensor method, electrochemical method, etc.), which provide references for the detection of strong polar pesticides and their metabolites residue.