响应面法优化黑胡萝卜红色素双水相萃取工艺

李治城; 武玉倩; 张婕妤; 展亚莉; 常秀莲

doi:10.13386/j.issn1002-0306.2020060219

响应面法优化黑胡萝卜红色素双水相萃取工艺

Optimization of Extraction Technology of Anthocyanidins from Daucus carota L. by Aqueous Two-phase System Based on Response Surface Methodology

摘要

摘要: 采用聚乙二醇（PEG）/硫酸铵双水相体系萃取纯化黑胡萝中的花青素。通过单因素实验考察了硫酸铵、PEG6000、粗提液的质量分数对花青素选择性系数和萃取率的影响，并以花青素的萃取率为响应值，结合响应面试验优化萃取工艺。结果表明，各因素对萃取率的影响程度由大到小依次为硫酸铵质量分数>粗提液质量分数>PEG6000质量分数。以双水相体系的总质量为基准，实验最佳萃取条件为：硫酸铵质量分数为20.0%、粗提液质量分数为12.0%、PEG6000质量分数为14.0%，黑胡萝卜红色素的平均萃取率为94.17%。此萃取方法得到的黑胡萝卜红色素具有一定的抗氧化活性，对DPPH自由基清除能力的IC₅₀值为30.51 mg/L。

Abstract: In this study, the anthocyanins in Daucus carota L. were extracted and purified by PEG/ammonium sulfate aqueous two-phase system. The mass content of ammonium sulfate, the mass content of PEG6000, and the mass content of crude extract were optimized by single factor experiment according to the selectivity coefficient and extraction ratio of anthocyanins. Based on the response surface experiment, with the extraction ratio of anthocyanins as the response value, the extraction process was optimized. The results showed that the influence order of various factors on anthocyanins extraction effect was as follows: the ammonium sulfate content>the crude extract content>the PEG6000 content. Based on the total mass of the aqueous two-phase system, the optimal extraction condition was as follows: the mass content of ammonium sulfate, crude extract and PEG6000 was 20.0%, 12.0% and 14.0%, respectively, and the average extraction ratio of black carrot red color was 94.17%. The black carrot red color extracted by this method had certain antioxidant activity, and the IC₅₀ value of the DPPH free radical scavenging ability was 30.51 mg/L.