响应面法优化大孔树脂纯化地黄多糖工艺

刘冲英; 周宁; 崔涛; 李瑾; 蒲定涛; 王国全; 张丽华; 赵鹏

doi:10.13386/j.issn1002-0306.2020050280

响应面法优化大孔树脂纯化地黄多糖工艺

Optimization of Purification Process of Polysaccharides from Rehmannia glutinosa Libosch. with Macroporous Adsorption Resins by Response Surface Methodology

摘要

摘要: 为研究大孔树脂纯化地黄多糖的最优工艺条件，以多糖保留率、脱色率、蛋白质脱除率的加权综合评分为指标选择具有较好纯化效果的大孔树脂，在单因素的实验基础上，根据响应面试验来优化地黄多糖的纯化工艺参数。结果表明，以HPD-400和LS-700B型大孔吸附树脂混合纯化地黄多糖效果最好，最佳纯化工艺为：树脂质量比（HPD-400：LS-700B）为1.20，上样浓度6.0 mg/mL，温度30℃，上样速率1.5 BV/h，上柱体积1.5 BV。纯化后的多糖保留率为90.25%，脱色率为83.15%，蛋白质脱除率79.75%，综合评分为84.97%。本研究得到的纯化工艺适用于地黄多糖纯化。

Abstract: The article was to investigate the optimal purification process for macroporous resins of polysaccharides from Rehmannia glutinosa Libosch. With weighted comprehensive scores of the rate of polysaccharide retention, decolorization and protein removal, the macroporous resin with better purification effect was selected. Then, the optimal experiments were carried out by single experiments and the Box-Behnken response surface methodology(RSM) experiment. The results showed that the mixture of HPD-400 and LS-700B macroporous resin had the best purification effect on Rehmannia glutinosa Libosch. polysaccharides, the optimal purification process was as follows: Resin mass ratio was 1.20, sample loading concentration 6.0 mg/mL, temperature 30℃, sample loading rate 1.5 BV/h, and the upper column volume 1.5 BV. After purification, the retention rate of polysaccharide was 90.25%, the decolorization rate was 83.15%, the protein removal rate was 79.75%, and the comprehensive scores was 84.97%. The purification process obtained in this study was suitable for the purification of Rehmannia glutinosa Libosch. polysaccharides.