Abstract: In order to study formulation optimization of konjac glucomannan(KGM)and ethyl cellulose(EC)composite film and its application to honey packaging,KGM,EC,and dibutyl glycerate(DBS)were used as raw materials,and water and ethyl acetate as the solvent to prepare films,and formulations optimization of the film was tested by the single factor and response surface methodology in conjunction with miscellaneous design. The result showed that the optimal formulation of the composite film was composed of 70% KGM,30% EC,and 8% DBS,and the prepared film had the maximum tensile strength of 64.56 MPa.Based on this,investigating the heat sealing conditions of KGM/EC film and applying the film to honey packaging,the result showed that the film reached a maximum sealing strength of 4.4 N/15 mm using 2% KGM sol as an adhesive to heat seal KGM/EC film for 14 s. The honey packaging bag was stored at 25 ° C and 50%±5% relative humidity for 22 days to achieve water vapor adsorption and desorption balance,and the weight loss rate was 9.3%. No microbial colonies in honey were detected. In summary,the KGM/EC composite film has potential application in honey packaging.