Abstract: Digital PCR(dPCR)technology is a rapid development of absolute quantification of nucleic acid molecules in recent years. It has the advantages of quantification without standard curve,high sensitivity and high accuracy. With the increasing requirements of detection technology in various fields,the application of digital PCR technology in the analysis of genetically modified products,animal and plants products,microorganisms and other fields has become more and more widespread. This article reviews the principles and applications of dPCR,analyzes its problems and prospects,and provides a reference for the application of digital PCR.