Abstract: Objective:To evaluate the effects of American Ginseng tea on immune function in SPF grade of BALB/c mice. Methods:BALB/c mice were randomly divided into control group,low-dose,medium-dose and high-dose American Ginseng tea group(0.275,0.550 and 1.100 g crude drug/kg,respectively),and American Ginseng soaking water group(0.500 g crude drug/kg). Experiments of spleen lymphocyte transformation,delayed type hypersensitivity,antibody-producing cell detection,serum hemolysin determination,carbon clearance,peritoneal macrophage phagocytosis fluorescent microspheres and NK cells activity were performed to observe the effect of American Ginseng tea on immune function of mice. And network pharmacology analysis was used to explore the mechanism of American ginseng tea. Results:American Ginseng tea had no significant effect on the weight gain and thymus coefficient of mice. Compared with the control group,the medium and high dose of Ginseng tea could significantly improve the cellular immunity and mononuclear macrophage clearance in mice. The high-dose group of American Ginseng tea could significantly improve spleen coefficient,humoral immune function and NK cell activity. And the low-dose group of American Ginseng tea could significantly increase the number of antibody-producing cells. β-sitosterol and ginsenoside Rh2 were considered to the major active substances in American Ginseng tea,and the mechanism of treatment may involve IL-17,NOD-like receptor and other inflammation-related pathways as well as apoptosis signaling pathways. Conclusion:American Ginseng tea has the potential to enhance immune function.