食源复方解酒口服液对小鼠酒精性肝损伤的保护作用

于纯淼; 付佳琪; 张良; 李治翔

doi:10.13386/j.issn1002-0306.2020040096

食源复方解酒口服液对小鼠酒精性肝损伤的保护作用

Protective Effect of Homology of Medicine and Food Compound Alleviate a Hangover Oral Liquid on Alcoholic Liver Injury in Mice

摘要

摘要: 目的：探讨食源复方解酒口服液对醉酒小鼠的解酒及对酒精性肝损伤小鼠的保肝作用。方法：将小鼠随机分成六组，正常组，模型组，阳性对照组（海王金樽1.30 mL/10 g），低剂量组、中剂量组、高剂量组（0.65、1.30、2.60 mL/10 g）。解酒作用实验：采用口服液对高、中、低剂量组进行灌胃干预，阳性对照组给予海王金樽，连续灌胃7 d，末次灌胃后30 min，以52°白酒灌胃，记录翻正反射消失和恢复时间。保肝作用研究：除正常组灌胃纯净水外、其余各组每天灌胃52°白酒，30 min后除正常组和模型组灌胃纯净水外，阳性对照组及高、中、低剂量组灌胃海王金樽和口服液，连续7 d。测量小鼠的体重变化并计算其肝脏系数，检测血清中谷丙转氨酶（Alanine aminotransferase，ALT）、谷草转氨酶（Aspartate aminotransferase，AST）及肝脏组织中乙醇脱氢酶（Alcohol dehydrogenase，ADH）和乙醛脱氢酶（Acetaldehyde dehydrogenase，ALDH）含量。结果：与模型组相比，口服液各剂量组可显著降低小鼠醉酒率、延长醉酒时间（P<0.05），缩短醒酒时间（P<0.05），其中口服液中、高剂量组还能明显降低AST（P<0.01）、ALT含量（P<0.01），低剂量组ALT含量虽低于模型组，但差异不明显（P>0.05）；口服液低剂量组能提高ADH活性（P<0.05），对ALDH效果不明显（P>0.05），而中、高剂量组能显著提高ADH（P<0.05）和ALDH（P<0.05）的活力水平。结论：食源复方解酒口服液具有解酒保肝作用。

Abstract: Objective:To explore the effect of Shiyuan Fufang alleviate a hangover oral liquid on the hangover effect of drunk mice and the liver-protecting effect of alcoholic liver injury mice. Methods:The mice were randomly divided into six groups,normal group,model group,positive control group(Neptune Gold Bottle 1.30 mL/10 g)low-dose group,medium-dose group,high-dose group(0.65,1.30,2.60 mL/10 g). Anti-alcoholic effect experiment:Gavage of high,medium and low dose groups with oral solution. The positive control group was given Neptune golden bottle for 7 days,and 30 minutes after the last intragastric administration,52 degrees of white spirits were used for intragastric administration. The regular reflection disappears and recovery time. Liver protection study:In addition to the normal group,the other groups were given with 52 degrees of white wine every day. After 30 minutes,in addition to the normal group and the model group,the positive control group and the high,medium and low dose groups Gavage Neptune Gold Bottle and oral liquid were consecutive given for 7 days. Measure the weight change of mice and calculate their liver coefficient,detect serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and liver tissue alcohol dehydrogenase(ADH)and acetaldehyde dehydrogenase(ALDH)content. Results:Compared with the model group,the oral solution of each dose group could significantly reduce the rate of drunken mice,prolong the drunken time(P<0.05),and shorten the time of hangover(P<0.05). Among them,the oral liquid medium and high dose groups could also significantly reduce AST(P<0.01),ALT content(P<0.01),although the ALT content in the low-dose group was lower than that in the model group,the difference was not obvious(P>0.05);The low-dose group in oral solution could increase ADH activity(P<0.05). The effect on ALDH was not obvious(P>0.05),while the medium and high dose groups could significantly increase the vitality level of ADH(P<0.05)and ALDH(P<0.05). Conclusion:Oral liquid has the effect of hangover and protect liver.