Abstract: In order to enrich EPA and DHA from pressed liquor of fish meal processing,the method of urea inclusion and molecular distillation were used,and the processing was optimized. The physicochemical indexes and fatty acid analysis of the enriched fish oil were carried out. The results showed that the ratio of urea to fish oil was 1.5:1,the ratio of solvent to fish oil was 6:1,the crystallization temperature was 0 ℃,the crystallization time was 15 h,and the inclusion times were once. The conditions of molecular distillation were as follows:Distillation temperature was 110 ℃,scraping speed was 250 r/min,flow rate was 3 mL/min,pressure was 5 Pa,and two level of molecular distillation was used. After urea inclusion and molecular distillation,the EPA and DHA content could increase from 22.68% to 87.50%,and EPA/DHA was about 0.58. The physicochemical indexes of enriched fish oil accorded with quality requirements for the first grade standard of refined fish oil.