Abstract: The objective of this work was to investigate the difference of heat-induced gel properties between black carp(Mylopharyngodon piceus)and silver carp(Hypophthalmichthys molitrix). The secondary structure,surface hydrophobicity(S₀-ANS),disulfide bonds,average particle size(d_z)and dynamic rheology of myosin during heat-induced gel formation were also studied. The results showed that denaturation degree of black carp myosin was higher than that of silver carp during the heat-induced gel formation,indicating more hydrophobic amino acid residues were exposed to form a higher hydrophobic interaction in black carp myosin. Therefore,the d_z of black carp myosin was about twice that of silver carp,and G' was higher than silver carp. The results showed that the myosin of black carp had a higher heat-induced gel forming ability than silver carp. The transformation of α-helix to β-fold or β-turn angle were conducive to the gelation of myosin.