紫苏籽蛋白对免疫力低下小鼠免疫调节功能的研究

朱艳; 姜盛; 李明亮; 董哲; 王雨晴; 郭颖; 谷瑞增

doi:10.13386/j.issn1002-0306.2020020189

紫苏籽蛋白对免疫力低下小鼠免疫调节功能的研究

Study on Immune Regulation of Perilla frutescens Seed Protein in Mice with Low Immunity

摘要

摘要: 目的:探究紫苏籽蛋白对免疫低下小鼠免疫系统的调节作用。方法:采用腹腔注射环磷酰胺(CTX)建立免疫低下小鼠模型,通过灌胃人体推荐剂量换算的低、中、高剂量的紫苏籽蛋白,检测其对小鼠脏器指数、T/B淋巴细胞增殖、血清溶血素、NK细胞杀伤力、吞噬指数、血清IL-2和IgG含量的影响。结果:紫苏籽蛋白对免疫低下小鼠免疫系统的调节效果随紫苏籽蛋白浓度增加呈先增加后降低趋势,中剂量的紫苏籽蛋白提高了免疫低下小鼠T/B淋巴细胞的增殖、半数溶血值(HC₅₀)、NK细胞杀伤力、巨噬细胞吞噬能力以及增加小鼠血清细胞因子IL-2含量,与模型组相比具有极显著差异(P<0.01),高剂量的紫苏籽蛋白免疫调节效果减弱。结论:适量摄入紫苏籽蛋白能发挥较强的免疫调节作用。

Abstract: Objective:To study the effect of Perilla frutescens seed protein on immune system regulation in immunocompromised mice. Methods:Cyclophosphamide(CTX)was injected intraperitoneally to establish a model of immunocompromised mice. The effects of low,medium and high doses of Perilla frutescens seed protein on visceral index,T/B lymphocyte proliferation,serum hemolysin,NK cell killing,phagocytosis index,serum IL-2 and IgG levels in mice were detected. Results:With the increasing of concentration of Perilla frutescens seed protein,the immunomodulatory effect of Perilla frutescens seed protein first increased and then decreased Medium dose of Perilla protein increased T/B lymphocyte proliferation,hemolytic value(HC50),NK cell lethality,macrophage phagocytosis,and serum cytokine IL-2 and IgG levels in immunocompromised mice,and there were significant differences compared with the model group(P<0.01). Conclusion:Perilla frutescens seed protein play a good role in immune regulation in immunocompromised mice.