基于DMSA自组装单分子层的SPR免疫传感器检测沙丁胺醇

李文杰; 王晨晨; 颜朦朦; 李慧冬; 丁蕊艳; 方丽萍; 王兰芝; 侯运华; 杨亲正; 陈子雷

doi:10.13386/j.issn1002-0306.2020020112

基于DMSA自组装单分子层的SPR免疫传感器检测沙丁胺醇

Detection of Salbutamol by SPR Immunosensor Based on DMSA Self-assembled Monolayers

摘要

摘要: 本文通过双巯基丁二酸(DMSA)作为单分子层连接表面等离子体共振(SPR)免疫传感器中芯片上的抗原,并以沙丁胺醇(SAL)为检测模型,构建了DMSA-SPR免疫传感器,将其检测效果与传统的巯基丙酸(MPA)-SPR传感器进行比较。结果表明,室温下,DMSA-SPR免疫传感器的抗原抗体识别效率增强近一倍,其线性范围达到5~150 ng/mL,定量限(LOQ)达到5 ng/mL。此传感器应用于猪肉中SAL检测的回收率达到94.9%~108.0%,并与超高效液相色谱-串联质谱仪(UPLC-MS/MS)检测结果具有很好的相关性。因此,该方法为提高SPR免疫传感器的灵敏度提供了新思路、新方法,并可广泛应用于其他小分子的检测。

Abstract: In this paper, dimercaptosuccinic acid (DMSA) was used as self-assembled monolayer to connect the antigen on the chip of surface plasmon resonance (SPR) immunosensor to construct DMSA-SPR immunosensor for detection of the model of salbutamol (SAL) and its detection effect was compared with that of traditional mercaptopropionic acid (MPA) -SPR sensor. The results showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was nearly doubled at room temperature, the linear range was up to 5~150 ng/mL, and the limit of quantification (LOQ) was 5 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9%~108.0%, and had a good correlation with the analysis results of ultra-high phase liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. Therefore, this method would provide a new idea and method for improving the sensitivity of SPR immunosensor, and could be widely used in the detection of other small molecules.