不同产地徐紫薯6号花青素成分差异分析

穆晶晶; 朱成雷; 徐静雯; 韩玮璐; 陈才法; 郑元林; 陈萍

doi:10.13386/j.issn1002-0306.2020010054

不同产地徐紫薯6号花青素成分差异分析

Difference Analysis of Anthocyanin Components from Xuzishu No.6 in Different Origins

摘要

摘要: 以徐紫薯6号为研究样本,建立了检测同一品种不同产地紫甘薯花青素含量与组分差异的高效液相色谱方法,并进行了指纹特征的聚类分析。采用1.5%磷酸和35%乙腈(1%磷酸)为流动相梯度洗脱,成功分离出分离度R>1,USP塔板系数达到10000以上的13种花青素组分,各组分的相对标准偏差(RSD)为0.42%~8.16%。同时分析了来自徐州(XZ₁₅)、睢宁(SN₁₅)、盐城(YC₁₅)三种植试点区的徐紫薯6号花青素含量。结果表明,徐紫薯6号花青素主要成分为矢车菊素-3-咖啡酰-p-羟基苯甲酰-槐糖苷-5-葡萄糖苷,其中XZ₁₅的总含量最高并含有13种花青素组成,样本YC₁₅的花青素主要含量提升,但组分大幅减少,样本SN₁₅的花青素总含量大幅度减少但保留全部13种花青素组分。聚类分析结果证明了三份样本花青素组成存在差异性,样本YC₁₅由于组成减少,主成分含量增加显示了与其它两者有较大的差异性。本实验建立的花青素鉴定方法分离效果好,灵敏度高,而不同地区徐紫薯6号花青素总含量和组成受到地域影响明显,可作为徐紫薯6号的产地溯源的指标之一,并对该品种在不同地区推广有指导作用。

Abstract: A determination method of anthocyanins in Xuzishu NO.6 by high performance liquid chromatography (HPLC) was established. 13 kinds of components with R>1 and over 1000 of USP theoretical plate number have been identified by this method which the resolution were 1.5% phosphoric acid and 35% acetonitrile (1% phosphoric acid), and the RSD was 0.42%~8.16%.The samples from XZ₁₅ (34°28'N 117°30'E), SN₁₅ (33°97'N 117°98'E), and YC₁₅ (33°00'N 120°82'E) were analyzed. The results revealed that the main component was cyanidin 3-caffeoyl-p-hydroxybenzoyl-sophoroside-5-glucoside.Whereas XZ₁₅ sample contained 13 kinds of components with highest content of total anthocyanins, YC₁₅ sample only contained 8 kinds of components but the highest main component contents. And SN₁₅ sample contained 13 kinds of components but low-content anthocyanins. The results of cluster analysis also showed that the anthocyanin composition differences among the 3 samples were relative to their origins, whereas YC₁₅ showed more differences because of its components reduce and main component contents increases. The method was well separated and proved to be sensitive and accurate. The results revealed that the anthocyanin composition was mostly affected by their origins. Therefore, this method is applied to geographical classification of Xuzishu No.6.