Abstract: Objective:To research the sleep improvement of Semen Ziziphi Spinosae,theanine and γ-aminobutyric acid formula products in mice. Methods:180 healthy male Balb-c mice were divided randomly into three groups. Each group was divided into four groups:One control group and three treatment group with equal size. Semen Ziziphi Spinosae,theanine and γ-aminobutyric acid were mixed in proportion and fed to mice at 95,190,285 mg/kg·bw respectively for 30 d by oral administration. Then direct sleep test,sleep extending test with sodium pentobarbital,hypnosis test under subthreshold dose of sodium pentobarbital and incubation period test with barbital sodium were conducted respectively. Results:The experimental samples had no direct hypnotic effect. The sleep time of mice injected with pentobarbital sodium in the low,medium and high dose group was longer by 14.53%,17.12%,23.80% compared with the model group(P<0.05). The rate of sleeping increased under the threshold hypnogenesis dosage of pentobarbital sodium in medium and high dose group(P<0.05). The sleeping delitescence induced by tobarbital sodium significant decreased by 12.48%,24.19%,24.07% compared with the model group(P<0.05). Conclusion:The formula consists of Semen Ziziphi Spinosae,theanine and γ-aminobutyric acid can shorten the sleep incubation period,prolong the sleep time and increase the sleeping rate of mice. According the results,the formula has the sleep improvement effect in mice.