副干酪乳杆菌K56调节肠道菌群及润肠通便功能研究

刘伟贤; 郝婧宇; 陈希民; 冷候喜; 王鑫; 李东; 赵雯; 赵子夫; 洪维鍊

doi:10.13386/j.issn1002-0306.2020.20.046

副干酪乳杆菌K56调节肠道菌群及润肠通便功能研究

Improvement in Intestinal Flora Regulation and Laxative Function to Lactobacillus paracasei K56

摘要

摘要: 目的:参考保健食品检验与技术评价规范(2003版)中关于调节肠道菌群及通便功能动物实验评价方法评价副干酪乳杆菌调节肠道菌群及润肠通便功能。方法:分别以0.000867、0.00867、0.0867、0.867、8.67 mg/kg灌胃给予副干酪乳杆菌K56菌株(实验室检测菌株规格为1.5×10¹¹ CFU/g),检测小鼠干预14 d前后双歧杆菌、乳杆菌、肠杆菌、肠球菌、产气荚膜梭菌含量,并测定干预30 d后小肠墨汁推进率、首粒黑便时间、排黑便粒数及重量,评价副干酪乳杆菌对小鼠肠道菌群及排便的影响。结果:干预14 d前后比较,各组小鼠粪便双歧杆菌、乳杆菌、产气荚膜梭菌均显著增加(P<0.05),而肠杆菌和肠球菌无显著变化(P>0.05);与对照组比较,以0.00867、0.0867、0.867 mg/kg剂量的K56干预后,小鼠肠道内双歧杆菌及乳杆菌数量显著增加(P<0.05),0.000867、0.00867、0.867 mg/kg剂量的K56干预后产气荚膜梭菌含量显著降低(P<0.05);与模型组比较,0.00867、0.867、8.67 mg/kg剂量组干预小鼠墨汁推进率显著增高(P<0.05),0.0867、0.867 mg/kg剂量组小鼠排黑便粒数与重量显著增加(P<0.05),但各剂量组对小鼠首粒黑便时间与模型对照组无显著性差异(P>0.05)。结论:副干酪乳杆菌K56菌株具有调节肠道菌群和润肠通便的作用,调节肠道菌群最佳剂量为0.0867 mg/kg(对应人体剂量1.0×10⁸ CFU/d),润肠通便最低有效剂量为0.867 mg/kg(对应人体剂量为1.0×10⁹ CFU/d)。

Abstract: Objective:To study the regulatory effect of a Lactobacillus paracasei K56 on intestinal flora and laxative function in mice according to the technical specification for health food inspection and evaluation in China(2003 edition).Methods:The mice were administered by gavage with 0.000867,0.00867,0.0867,0.867,8.67 mg/kg Lactobacillus paracasei K56(The specification was 1.5×10¹¹ CFU/g)and the amounts of Bifidobacteria,Lactobacilli,Enterobacter,Enterococci,and Clostridium perfringens were detected before and after mice administered with Lactobacillus paracasei K56 for 14 d. After intervention the mice were sacrificed and movement rate of black ink in the small intestine,time of the first evacuation of black stool,and number and weight of black fecal pellets were measured. Results:The control group and all intervention groups had higher abundance in Bifidobacteria,Lactobacilli,and C. perfringens in the feces after 14 d(P<0.05)and no significant differences for Enterobacter and Enterococcus species(P>0.05). Compared to the normal control group,the numbers of Bifidobacteria and Lactobacilli increased significantly after receiving 0.00867,0.0867,0.867 mg/kg. L. paracasei K56(P<0.05),although the number of C. perfringens significantly raised in the 0.000867,0.00867,0.867 mg/kg groups(P<0.05). Compared with model group,the movement rate of ink in the small intestine increased significantly in group receiving 0.00867,0.867,8.67 mg/kg. L. paracasei K56 per day(P<0.05),and the numbers and weight of black fecal pellets were more in groups of 0.0867,0.867 mg/kg(P<0.05).The time of the first evacuation of black stool in all groups were not significantly different. Conclusion:This study verified that the probiotic strain Lactobacillus paracasei K56 exerted beneficial effect on regulating intestinal flora and relaxing bowel,implying its use as a functional ingredient in food products. The most effective dose of K56 on regulating intestinal flora was 0.0867 mg/kg(Person dose was 1.0×10⁸ CFU/d)and the minimum effective dose for the laxative function was 0.867 mg/kg(Person dose was 10⁹ CFU/g).