Abstract: A rapid method to detect six kinds of Alternaria toxins(including alternariol mono-methyl ether,altenuene,tenuazonic acid,alternariol,tentoxin and altenusin)in mango by ultra-high performance liquid chromatography-mass spectrometry was established. Further samples were extracted and purificated by improved QuEChERS method. The mango samples with peel and without peel were used as the matrix. A proper amount of water was added to the mango samples.1.5% formic acid acetonitrile solution as the extraction agent,anhydrous MgSO₄ as the dehydrating agent,NaCl as salting out,which were shaked,mixed and centrifuged at 9500 r/min for 5 min. Finally the supernatant was diluted with water and separated by Waters ACQUITYTM UPLC BEH C₁₈ column(1.7 μm,2.1 mm×100 mm)and monitored by electrospray ionization positive ion multi reaction mode. Under these conditions,the linear relationship of six kinds of Alternaria toxins was good in the concentration range of 0.5~200 ng/mL,R²>0.9925.The detection limit was 0.6~3.0 μg/kg when S/N was 3.The recoveries of six Alternaria toxins were 82.5%~110.6% and the relative standard deviations was less than 10% at 5,10,20 μg/kg. This method is simple and efficient,which could be used to detect six Alternaria toxins in mango.