翠云草挥发油成分分析、抗氧化及抗菌效果

雷杰; 黎维维; 欧阳陈琳; 马先奎; 王刚

doi:10.13386/j.issn1002-0306.2020.17.045

翠云草挥发油成分分析、抗氧化及抗菌效果

Composition Analysis,Antioxidant and Antibacterial Effect of Volatile Oil from Selaginella uncinata

摘要

摘要: 目的:对翠云草挥发油进行成分分析、抗氧化及抗菌研究。方法:采用气相色谱-质谱仪(GC-MS)对翠云草挥发油化学成分进行分离鉴定;采用DPPH法,FRAP法和金属离子螯合能力实验评价翠云草挥发油的体外抗氧化活性;用纸片扩散法测定翠云草挥发油的抗菌活性,包括3种革兰氏阳性菌,即金黄色葡萄球菌、粪肠球菌、化脓棒状杆菌,和3种革兰氏阴性菌,即大肠杆菌、铜绿假单胞菌、变形杆菌。结果:从翠云草中分离出52个峰,鉴定出50种成分,已鉴定成分占总面积的98.58%,主要成分有植酮(21.40%)、角鲨烯(8.53%)、棕榈酸(6.71%)、二十八烷(6.03%)等。翠云草具有潜在的抗氧化活性,DPPH自由基清除的IC₅₀值为0.76 mg/mL,铁离子还原能力的FRAP值为0.86 mmol/L,金属离子螯合作用的EC₅₀值为0.71 mg/mL。通过抗菌实验的MIC和MBC结果表明翠云草挥发油对粪肠球菌、化脓棒状杆菌、大肠杆菌和铜绿假单胞菌则具有较好的抗菌活性,其中对粪肠球菌的MIC值为5 μL/mL,MBC值为10 μL/mL;对化脓棒状杆菌的MIC值为10 μL/mL,MBC值为20 μL/mL。结论:本文通过GC-MS分析了翠云草挥发油成分,并首次发现其具有一定的抗氧化活性及抗菌活性。

Abstract: Objective:To analyze the constituents,anti-oxidant and antibacterial properties of volatile oil from Selaginella uncinata. Methods:GC-MS was used to separate and identify the chemical components of the volatile oil of Selaginella uncinata. DPPH,FRAP and chelation of metal ions were used to evaluate the antioxidant activity of the volatile oil of Selaginella uncinata in vitro. Paper diffusion method was used to determine the antibacterial activity of volatile oil of Selaginella uncinata,including three Gram-positive bacteria,namely Staphylococcus aureus,Enterococcus faecalis and Corynebacterium pyogenes,and three kinds of Gram-negative bacteria,namely Escherichia coli,Pseudomonas aeruginosa and proteus. Results:52 peaks were isolated and 50 components were identified. The identified components accounted for 98.58% of the total area. The main components were fitone(21.40%),squalene(8.53%),palmitic acid(6.71%),octacosane(6.03%). The IC₅₀ value of DPPH was 0.76 mg/mL,FRAP value of iron ion reduction was 0.86 mmol/L,and EC₅₀ value of metal ion chelation was 0.71 mg/mL. The results of MIC and MBC showed that the volatile oil of Selaginella uncinata had good antibacterial activity against Enterococcus faecalis,Corynebacterium pyogenes,Escherichia coli and Pseudomonas aeruginosa. The MIC value of Enterococcus faecalis was 5 μL/mL,MBC value was 10 μL/mL;the MIC value of Corynebacterium pyogenes was 10 μL/mL,MBC value was 20 μL/mL. Conclusion:In this paper,the volatile oil components of Selaginella uncinata were analyzed by GC-MS,and it was found for the first time that it had certain antioxidant activity and antibacterial activity.