Abstract: An analytical method was developed for determination of six kinds of eugenol drug residues in aquatic products at the same time by using DMSO assisted concentration and GC-MS. Sample was ultrasonic extracted by acetonitrile after adding anhydrous sodium sulfate,degreased using acetonitrile saturated n-hexane. The acetonitrile layer was added with DMSO for nitrogen blowing to nearly dry and determined by GC-MS using DB-1701.Six analytes had good relationships in concentration range of 20~1000 μg/L,the correlation coefficients were not less than 0.999,and the average recovery rates under the three different concentrations were 64.8%~110.9%(n=6).This method was simple,rapid,with good repeatability,good stability and good accuracy,and reduced analyte losses in nitrogen blowing significantly. The method was suitable for the determination of clove phenol drug residues in aquatic products and qualitative confirmation research.