Abstract: A rapid method for the determination of paclitaxel in health care wine using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was developed. The sample was diluted with water for 10 times,cleaned up by GCB SPE column,and loaded onto Luna C₈(150 mm×2 mm,3 μm)using a mobile phase gradient with 0.2% formic acid and acetonitrile. The determination was carried out with electrospray ion source under the positive(ESI⁺)and multiple-reaction monitoring(MRM)mode. The calibration curves showed a good linearity in the range of 1~100 μg/L,and the coefficients of determination(R²)were higher than 0.999. The limits of quantification(LOQ,S/N=10)of paclitaxel was 10 μg/L for the health care wine. The average recoveries were from 88.9% to 92.4%,and the relative standard deviation(RSD)was from 7.8% to 9.5% at the spiked levels of 10.0,50.0 and 100.0 μg/L in health care wine(n=6). Seventeen batches of health wine samples were detected using the method,two batches of samples were detected,and the contents were all more than 100 times of the quantitative limit of the method.The method proved to be simple,efficient,sensitive and accurate,and could be used to detect the paclitaxel in health care wine. It would provide technical support for law enforcement agencies to supervise the illegal act of adding Taxus in health wine.