Abstract: In this study,extraction of isoquercitrin,astragaloside and rutin from mulberry leaves by enzymatic hydrolysis with compound enzymes(laccase,cellulase,hemicellulase and papain)were studied. Taking isoquercetin as the response value,the optimal conditions of extracting flavonoids from mulberry leaves,such as enzymolysis time,enzymolysis activation temperature,compound enzyme dosage and pH were optimized by uniform design method,and the interaction among the appropriate conditions was analyzed.The results showed that when the mass of mulberry leaves was 2 g,the dosage of complex enzyme was 0.675 g,the ratio of solid to liquid was 1:30,the isoquercetin 3.45 mg,rutin 1.45 mg and astragaloside 1.58 mg of mulberry leaves could be extracted at the pH value of 4.3 and 35℃ water bath temperature for 30 min,which was 60.37%,43.42% and 24.74% higher than alcohol extraction under the same conditions,respectively. It was found that the functional groups of C-H,-CH₂ and O-H of xylan,-C≡C-,>C=C