Abstract: Using the HPMC-Pulullan as the main component and the κ-carrageenan as the gelling agent,the influence of different composition ratios on performance indicators was investigated,and then the optimum formula was to be determined. According to the single-factor experiments,the formula design of the composite film was optimized by using the response surface methodology,and then the best preparation process was determined:The contents of pullulan was 9.533%,the contents of HPMC was 6.000%,the contents of κ-carrageenan was 2.315%,and the contents of glycerol was 7.903%. Under these conditions,the experimental results showed that the elongation at break of composite membrane was 56.35%±1.48%,the tensile strength was(2.68±0.39) MPa,the jelly strength was(785.48±10.32) g/cm²,and the light transmittance was 46.73%±3.87%. The experimental verification results were basically consistent with the theoretical values. Therefore,the results obtained by response surface optimization show certain guiding significance for the actual production and in-depth research of composite membrane.